Lipopolysaccharide induces Ca2+-independent nitric oxide synthase activity in rat gastric mucosal cells

James F. Brown, Barry L. Tepperman, Peter J. Hanson*, Brendan J. R. Whittle

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review


Ca2+-independent nitric oxide synthase was detected in gastric mucosal cells isolated from rats injected 4 h previously with Escherichia coli lipopolysaccharide (3 mg/kg i.v.). Induced nitric oxide synthase was located in an elutriated cell fraction of intermediate size which contained epithelial cells, but was absent from the parietal cell fraction. Administration of dexamethasone (2 mg/kg i.p.) 1 h before lipopolysaccharide inhibited the appearance of Ca2+-independent nitric oxide synthase, and prevented the observed reduction in cell viability (trypan blue exclusion). Ca2+-independent nitric oxide synthase activity can thus be induced in certain cells of the gastric mucosa, and may contribute to gastric pathologies where there is activation of the immune system.

Original languageEnglish
Pages (from-to)111-114
Number of pages4
JournalEuropean Journal of Pharmacology: Environmental Toxicology and Pharmacology
Issue number1
Publication statusPublished - 1 Nov 1994


  • (Stomach)
  • Gastric mucosa
  • Lipopolysaccharide
  • Nitric oxide (NO)


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