Long-chain n — 3 polyunsaturated fatty acid production by members of the marine protistan group the thraustochytrids: screening of isolates and optimisation of docosahexaenoic acid production

R. Bowles, A. Hunt, Graham Bremer, M. Duchars, R. Eaton

Research output: Chapter in Book/Report/Conference proceedingChapter (peer-reviewed)peer-review

Abstract

Following an isolation programme for thraustochytrids (marine fungoid protists) from three different locations, 57 isolates were screened for biomass, oil and docosahexaenoic acid production (DHA). Although a common fatty acid profile for the thraustochytrid isolates emerged, there was considerable variation in the DHA content of the oil. In some isolates from a cold temperate environment, DHA represented almost 50% of the total fatty acids present. Although isolates from a sub-tropical environment produced higher levels of biomass, with up to 37% (w/w) oil, the DHA fraction of the fatty acids was low. Cool temperate isolates gave intermediate values. Studies to optimise biomass and DHA production by manipulation of growth medium composition were carried out on a tropical strain. Results indicated that medium with a high C:N ratio stimulated DHA production. The use of such media in bioreactor cultivations gave maximum biomass, lipid and DHA content of 14 g l− 1, 78 and 25% (w/w), respectively. Optimum DHA production was 2.17 g l−1 after 107 h cultivation.
Original languageEnglish
Title of host publicationMarine bioprocess engineering, proceedings of an International Symposium organized under auspices of The Working Party on Applied Biocatalysis of the Eurpean Federation of Biotechnology and The European Society for Marine Biotechnology
EditorsR. Osinga, J. Tramper, J. Burgess, R. Wijffels
Place of PublicationThe Netherlands
PublisherElsevier
Pages193-202
Number of pages10
Volume35
ISBN (Print)9780444503879
DOIs
Publication statusPublished - 1999

Publication series

NameProgress in industrial microbiology
PublisherElsevier

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