Mesenchymal stem cells with increased stromal cell-derived factor 1 expression enhanced fracture healing

Chih-Yuan Ho, Anita Sanghani, Jia Hua, Melanie Coathup, Priya Kalia, Gordon Blunn

Research output: Contribution to journalArticlepeer-review

101 Downloads (Pure)


Treatment of critical size bone defects pose a challenge in orthopedics. Stem cell therapy together with cytokines has the potential to improve bone repair as they cause the migration and homing of stem cells to the defect site. However, the engraftment, participation, and recruitment of other cells within the regenerating tissue are important. To enhance stem cell involvement, this study investigated overexpression of stem cells with stromal cell-derived factor 1 (SDF-1) using an adenovirus. We hypothesized that these engineered cells would effectively increase the migration of native cells to the site of fracture, enhancing bone repair. Before implantation, we showed that SDF-1 secreted by transfected cells increased the migration of nontransfected cells. In a rat defect bone model, bone marrow mesenchymal stem cells overexpressing SDF-1 showed significantly (p=0.003) more new bone formation within the gap and less bone mineral loss at the area adjacent to the defect site during the early bone healing stage. In conclusion, SDF-1 was shown to play an important role in accelerating fracture repair and contributing to bone repair in rat models, by recruiting more host stem cells to the defect site and encouraging osteogenic differentiation and production of bone.

Original languageEnglish
Pages (from-to)594-602
Number of pages9
JournalTissue Engineering - Part A
Issue number3-4
Publication statusPublished - Feb 2015


  • Animals
  • Cell Differentiation
  • Chemokine CXCL12
  • Female
  • Femoral Fractures
  • Fracture Healing
  • Male
  • Mesenchymal Stem Cell Transplantation
  • Mesenchymal Stromal Cells
  • Osteogenesis
  • Rats
  • Rats, Wistar
  • Up-Regulation


Dive into the research topics of 'Mesenchymal stem cells with increased stromal cell-derived factor 1 expression enhanced fracture healing'. Together they form a unique fingerprint.

Cite this