Methods for the analysis of protein-chromatin interactions

S. Brickwood, Fiona Myers, S. Chandler

    Research output: Contribution to journalArticlepeer-review

    Abstract

    The analysis of protein interactions with chromatin is vital for the understanding of DNA sequence recognition in vivo. Chromatin binding requires the interaction of proteins with DNA lying on the macromolecular protein surface of nucleosomes, a situation that can alter factor binding characteristics substantially when compared with naked DNA. It is therefore important to study these protein-DNA interactions in the context of a chromatin substrate, the more physiologically relevant binding situation. In this article we review techniques used in the investigation of protein interactions with defined nucleosomal templates.
    Original languageEnglish
    Pages (from-to)1-15
    Number of pages15
    JournalMolecular Biotechnology
    Volume20
    Issue number1
    DOIs
    Publication statusPublished - 2002

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