Microsatellite markers development in the crop wild relative Linum bienne Mill. (Linaceae) using genome skimming

Beatrice Landoni; Juan Viruel; Rocio Gómez; Robin G. Allaby; Adrian, C. Brennan; F. Xavier Picó; Rocio Perez-Barrales

doi:10.1002/aps3.11349

Microsatellite markers development in the crop wild relative Linum bienne Mill. (Linaceae) using genome skimming

Beatrice Landoni, Juan Viruel, Rocio Gómez, Robin G. Allaby, Adrian, C. Brennan, F. Xavier Picó, Rocio Perez-Barrales

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Abstract

Premise: Nuclear microsatellite markers were developed for Linum bienne , the sister species of the crop L. usitatissimum , to provide molecular genetic tools for the investigation of L. bienne genetic diversity and structure.

Methods and Results: Fifty microsatellite loci were identified in L. bienne by means of genome skimming, and 44 loci successfully amplified. Of these, 16 loci evenly spread across the L. usitatissimum reference nuclear genome were used for genotyping six L. bienne populations. Excluding one monomorphic locus, the number of alleles per locus ranged from two to 12. Four out of six populations harbored private alleles. The levels of expected and observed heterozygosity were 0.076 to 0.667 and 0.000 to 1.000, respectively. All 16 loci successfully cross‐amplified in L. usitatissimum.

Conclusions: The 16 microsatellite loci developed here can be used for population genetic studies in L. bienne , and 28 additional loci that successfully amplified are available for further testing.

Original language	English
Article number	e11349
Number of pages	6
Journal	Applications in Plant Sciences
Volume	8
Issue number	5
DOIs	https://doi.org/10.1002/aps3.11349
Publication status	Published - 26 May 2020

Keywords

crop wild relative
Linaceae
Linum bienne
pale flax
population genetics
simple sequence repeat (SSR)

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Microsatellite markers development publishedFinal published version, 312 KBLicence: CC BY

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title = "Microsatellite markers development in the crop wild relative Linum bienne Mill. (Linaceae) using genome skimming",

abstract = "Premise: Nuclear microsatellite markers were developed for Linum bienne , the sister species of the crop L. usitatissimum , to provide molecular genetic tools for the investigation of L. bienne genetic diversity and structure.Methods and Results: Fifty microsatellite loci were identified in L. bienne by means of genome skimming, and 44 loci successfully amplified. Of these, 16 loci evenly spread across the L. usitatissimum reference nuclear genome were used for genotyping six L. bienne populations. Excluding one monomorphic locus, the number of alleles per locus ranged from two to 12. Four out of six populations harbored private alleles. The levels of expected and observed heterozygosity were 0.076 to 0.667 and 0.000 to 1.000, respectively. All 16 loci successfully cross‐amplified in L. usitatissimum.Conclusions: The 16 microsatellite loci developed here can be used for population genetic studies in L. bienne , and 28 additional loci that successfully amplified are available for further testing.",

keywords = "crop wild relative, Linaceae, Linum bienne, pale flax, population genetics, simple sequence repeat (SSR)",

author = "Beatrice Landoni and Juan Viruel and Rocio G{\'o}mez and Allaby, {Robin G.} and Brennan, {Adrian, C.} and Pic{\'o}, {F. Xavier} and Rocio Perez-Barrales",

year = "2020",

month = may,

day = "26",

doi = "10.1002/aps3.11349",

language = "English",

volume = "8",

journal = "Applications in Plant Sciences",

issn = "2168-0450",

publisher = "Botanical Society of America Inc.",

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TY - JOUR

T1 - Microsatellite markers development in the crop wild relative Linum bienne Mill. (Linaceae) using genome skimming

AU - Landoni, Beatrice

AU - Viruel, Juan

AU - Gómez, Rocio

AU - Allaby, Robin G.

AU - Brennan, Adrian, C.

AU - Picó, F. Xavier

AU - Perez-Barrales, Rocio

PY - 2020/5/26

Y1 - 2020/5/26

N2 - Premise: Nuclear microsatellite markers were developed for Linum bienne , the sister species of the crop L. usitatissimum , to provide molecular genetic tools for the investigation of L. bienne genetic diversity and structure.Methods and Results: Fifty microsatellite loci were identified in L. bienne by means of genome skimming, and 44 loci successfully amplified. Of these, 16 loci evenly spread across the L. usitatissimum reference nuclear genome were used for genotyping six L. bienne populations. Excluding one monomorphic locus, the number of alleles per locus ranged from two to 12. Four out of six populations harbored private alleles. The levels of expected and observed heterozygosity were 0.076 to 0.667 and 0.000 to 1.000, respectively. All 16 loci successfully cross‐amplified in L. usitatissimum.Conclusions: The 16 microsatellite loci developed here can be used for population genetic studies in L. bienne , and 28 additional loci that successfully amplified are available for further testing.

AB - Premise: Nuclear microsatellite markers were developed for Linum bienne , the sister species of the crop L. usitatissimum , to provide molecular genetic tools for the investigation of L. bienne genetic diversity and structure.Methods and Results: Fifty microsatellite loci were identified in L. bienne by means of genome skimming, and 44 loci successfully amplified. Of these, 16 loci evenly spread across the L. usitatissimum reference nuclear genome were used for genotyping six L. bienne populations. Excluding one monomorphic locus, the number of alleles per locus ranged from two to 12. Four out of six populations harbored private alleles. The levels of expected and observed heterozygosity were 0.076 to 0.667 and 0.000 to 1.000, respectively. All 16 loci successfully cross‐amplified in L. usitatissimum.Conclusions: The 16 microsatellite loci developed here can be used for population genetic studies in L. bienne , and 28 additional loci that successfully amplified are available for further testing.

KW - crop wild relative

KW - Linaceae

KW - Linum bienne

KW - pale flax

KW - population genetics

KW - simple sequence repeat (SSR)

UR - https://bsapubs.onlinelibrary.wiley.com/doi/full/10.1002/aps3.11349

U2 - 10.1002/aps3.11349

DO - 10.1002/aps3.11349

M3 - Article

SN - 2168-0450

VL - 8

JO - Applications in Plant Sciences

JF - Applications in Plant Sciences

IS - 5

M1 - e11349

ER -

Microsatellite markers development in the crop wild relative Linum bienne Mill. (Linaceae) using genome skimming

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Keywords

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