Modification of titanium alloy surfaces for percutaneous implants by covalently attaching laminin

David J Gordon, Dimpu D Bhagawati, Catherine J Pendegrass, Claire A Middleton, Gordon W Blunn

    Research output: Contribution to journalArticlepeer-review

    Abstract

    Percutaneous implants require a seal at the skin interface. Laminin (L-332) is a component of the basement membrane, integral to epidermal attachment. To enhance the attachment of keratinocytes onto the surface of titanium alloy (Ti(6)Al(4)V), we attached L-332 onto the surface using silanization (L-332==Ti(6)Al(4)V). Iodinated L-332 was used to investigate protein attachment kinetics. L-332==Ti(6)Al(4)V remained attached after immersion in serum compared with adsorbed L-332. Cells from a keratinocyte line (HaCaT) grown on L-332==Ti(6)Al(4)V were significantly smaller (p < 0.05) with over a 20-fold increase in the number of adhesion plaques compared with nontreated Ti(6)Al(4)V control discs or with adsorbed L-332 surfaces. We have shown that it is possible to augment the surface of Ti(6)Al(4)V with L-332 and that this significantly increases the attachment of keratinocytes.

    Original languageEnglish
    Pages (from-to)586-93
    Number of pages8
    JournalJournal of Biomedical Materials Research Part A
    Volume94
    Issue number2
    DOIs
    Publication statusPublished - Aug 2010

    Keywords

    • Alloys
    • Biocompatible Materials
    • Cell Adhesion
    • Cell Line
    • Cell Proliferation
    • Humans
    • Keratinocytes
    • Laminin
    • Materials Testing
    • Prostheses and Implants
    • Proteins
    • Surface Properties
    • Titanium
    • Vinculin

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