Nanopore sequencing for mixed samples

Research output: Chapter in Book/Report/Conference proceedingChapter (peer-reviewed)peer-review


Long read Nanopore sequencing can be utilised to determine the quality and accuracy of genetically engineered changes in animals, which often produce heterogenous samples. The protocol presented in this chapter can be used for a range of both low and high throughput sequencing applications. DNA must be repaired, barcoded and ligated to sequencing adapters prior to sequencing. Quality of sequencing data produced is dependent on stringent adherence to the protocol. However, nanopore sequencing is a fast moving field, therefore it is worth considering using the most up to date chemistry available.
Original languageEnglish
Title of host publicationDNA Manipulation and Analysis
EditorsGarry Scarlett
Place of PublicationNew York
PublisherHumana Press
Number of pages22
ISBN (Electronic)9781071630044
ISBN (Print)9781071630037
Publication statusPublished - 1 Mar 2023

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029


  • next-generation sequencing
  • third-generation nanopore sequencing
  • library preparation
  • Oxford nanopore technologies

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