Nanopore sequencing for mixed samples

Research output: Chapter in Book/Report/Conference proceedingChapter (peer-reviewed)peer-review

Abstract

Long read Nanopore sequencing can be utilised to determine the quality and accuracy of genetically engineered changes in animals, which often produce heterogenous samples. The protocol presented in this chapter can be used for a range of both low and high throughput sequencing applications. DNA must be repaired, barcoded and ligated to sequencing adapters prior to sequencing. Quality of sequencing data produced is dependent on stringent adherence to the protocol. However, nanopore sequencing is a fast moving field, therefore it is worth considering using the most up to date chemistry available.
Original languageEnglish
Title of host publicationDNA Manipulation and Analysis
EditorsGarry Scarlett
Place of PublicationNew York
PublisherHumana Press
Chapter16
Pages213-234
Number of pages22
ISBN (Electronic)9781071630044
ISBN (Print)9781071630037
DOIs
Publication statusPublished - 1 Mar 2023

Publication series

NameMethods in Molecular Biology
Volume2633
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • next-generation sequencing
  • third-generation nanopore sequencing
  • library preparation
  • Oxford nanopore technologies

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