TY - JOUR
T1 - Non-destructive genome skimming for aquatic copepods
AU - Vakati, Vinod
AU - Dodsworth, Steven
N1 - Funding Information:
We thank the editor and an anonymous reviewer for their valuable comments. We acknowledge the support from the Scientific Research Project of Neijiang Normal University (Grant No. 15ZL04) and the Major Scientific and Technological Achievements Transformation Project of Neijiang Normal University (Grant No. 16CZ04).
Funding Information:
We thank the editor and an anonymous reviewer for their valuable comments. We acknowledge the support from the Scientific Research Project of Neijiang Normal University (Grant No. 15ZL04) and the Major Scientific and Technological Achievements Transformation Project of Neijiang Normal University (Grant No. 16CZ04).
Publisher Copyright:
© 2020, The Author(s).
PY - 2020/9/1
Y1 - 2020/9/1
N2 - Copepods are important ecologically and represent a large amount of aquatic biomass in both freshwater and marine systems. Despite this, the taxonomy of copepods and other meiofauna is not well understood, hampered by tiny sizes, cryptic taxa, intraspecific polymorphisms and total specimen destruction where DNA methods are employed. In this article we highlight these issues and propose a more up-to-date approach for dealing with them. Namely, we recommend non-destructive DNA extraction methods, coupled with high-throughput sequencing (HTS). Whilst DNA yields may be low, they should still be sufficient for HTS library preparation and DNA sequencing. At the same time morphological specimens can be preserved and the crucial link between morphology and DNA sequence is maintained. This is critical for an integrative taxonomy and a fuller understanding of biodiversity patterns as well as evolutionary processes in meiofauna.
AB - Copepods are important ecologically and represent a large amount of aquatic biomass in both freshwater and marine systems. Despite this, the taxonomy of copepods and other meiofauna is not well understood, hampered by tiny sizes, cryptic taxa, intraspecific polymorphisms and total specimen destruction where DNA methods are employed. In this article we highlight these issues and propose a more up-to-date approach for dealing with them. Namely, we recommend non-destructive DNA extraction methods, coupled with high-throughput sequencing (HTS). Whilst DNA yields may be low, they should still be sufficient for HTS library preparation and DNA sequencing. At the same time morphological specimens can be preserved and the crucial link between morphology and DNA sequence is maintained. This is critical for an integrative taxonomy and a fuller understanding of biodiversity patterns as well as evolutionary processes in meiofauna.
KW - DNA barcoding
KW - genome skimming
KW - high-throughput sequencing
KW - mitogenomics
KW - non-destructive DNA extraction
KW - systematics
UR - http://www.scopus.com/inward/record.url?scp=85078170210&partnerID=8YFLogxK
UR - https://uobrep.openrepository.com/handle/10547/623947
U2 - 10.1007/s12686-020-01129-9
DO - 10.1007/s12686-020-01129-9
M3 - Article
AN - SCOPUS:85078170210
SN - 1877-7252
VL - 12
SP - 515
EP - 520
JO - Conservation Genetics Resources
JF - Conservation Genetics Resources
IS - 3
ER -
