TY - JOUR
T1 - Novel microsatellite markers for Dalechampia scandens (Euphorbiaceae) and closely related taxa
T2 - application to studying a species complex
AU - Falahati-Anbaran, Mohsen
AU - Stenoien, Hans
AU - Bolstad, Geir H.
AU - Hansen, Thomas F.
AU - Perez-Barrales, Rocio
AU - Armbruster, Scott
AU - Pelabon, Christophe
PY - 2017/4
Y1 - 2017/4
N2 - We developed novel microsatellite markers for Dalechampia scandens L. (Euphorbiaceae). The target plants belong to a distinct, but undescribed, species in the D. scandens species complex, characterized by small resin-producing glands. In total, 110 alleles over 36 novel markers were identified across 39 individuals from three populations. The number of alleles varied from one to seven, with an average of 3.06 alleles per locus. The developed markers, along with previously developed ones for large-glanded D. scandens species, were tested for amplification in 11 additional species of the genus Dalechampia. Four markers did not produce any detectable allele in 37 individuals from two populations of the large-glanded species. Average population genetic diversity for small- and large-glanded populations was 0.20 and 0.43, respectively. Cross-species amplification showed that 89% of all markers were successfully amplified in at least one of the eleven other Dalechampia species. These microsatellite markers may be useful for detecting undescribed species in the D. scandens species complex, and can be used for comparative analyses of genetic structure, mating system, and phylogeography of other Dalechampia species.
AB - We developed novel microsatellite markers for Dalechampia scandens L. (Euphorbiaceae). The target plants belong to a distinct, but undescribed, species in the D. scandens species complex, characterized by small resin-producing glands. In total, 110 alleles over 36 novel markers were identified across 39 individuals from three populations. The number of alleles varied from one to seven, with an average of 3.06 alleles per locus. The developed markers, along with previously developed ones for large-glanded D. scandens species, were tested for amplification in 11 additional species of the genus Dalechampia. Four markers did not produce any detectable allele in 37 individuals from two populations of the large-glanded species. Average population genetic diversity for small- and large-glanded populations was 0.20 and 0.43, respectively. Cross-species amplification showed that 89% of all markers were successfully amplified in at least one of the eleven other Dalechampia species. These microsatellite markers may be useful for detecting undescribed species in the D. scandens species complex, and can be used for comparative analyses of genetic structure, mating system, and phylogeography of other Dalechampia species.
KW - cryptic biodiversity
KW - species complex
KW - microsatellite loci
KW - Euphorbiaceae
KW - Dalechampia
KW - cross-species amplification
U2 - 10.1111/1442-1984.12142
DO - 10.1111/1442-1984.12142
M3 - Article
SN - 1442-1984
VL - 32
SP - 179
EP - 186
JO - Plant Species Biology
JF - Plant Species Biology
IS - 2
ER -