Parameter optimization for thermostable lipase production and performance evaluation as prospective detergent additive

Rajesh Kumar Sahoo; Aradhana Das; Mahendra Gaur; Anshuman  Sahu; Saubhagini Sahoo; Suchanda  Dey; Pattanathu Rahman; Enketeswara Subudhi

doi:10.1080/10826068.2020.1719513

Parameter optimization for thermostable lipase production and performance evaluation as prospective detergent additive

Rajesh Kumar Sahoo, Aradhana Das, Mahendra Gaur, Anshuman Sahu, Saubhagini Sahoo, Suchanda Dey, Pattanathu Rahman, Enketeswara Subudhi

School of Biological Sciences

Siksha O Anusandhan

Research output: Contribution to journal › Article › peer-review

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Abstract

Lipase based formulations has been a rising interest to laundry detergent industry for their ecofriendly property over phosphate-based counterparts and compatibility with chemical detergents ingredients. A thermo-stable Anoxybacillus sp. ARS-1 isolated from Taptapani Hotspring, India was characterized for optimum lipase production employing statistical model central composite design (CCD) under four independent variables (temperature, pH, % moisture and bio-surfactant) by solid substrate fermentation (SSF) using mustard cake. The output was utilized to find the effect of parameters and their interaction employing response surface methodology (RSM). A quadratic regression with R2¼0.955 established the model to be statically best fitting and a predicted highest lipase production of 29.4IU/g at an optimum temperature of 57.5oC, pH 8.31, moisture 50% and 1.2mg of bio-surfactant. Experimental production of 30.3IU/g lipase at above conditions validated the fitness of model. Anoxybacillus sp. ARS-1 produced lipase was found to resist almost all chemical detergents as well as common laundry detergent, proving it to be a prospective additive for incorporation.

Original language	English
Number of pages	7
Journal	Preparative Biochemistry and Biotechnology
Early online date	3 Feb 2020
DOIs	https://doi.org/10.1080/10826068.2020.1719513
Publication status	Early online - 3 Feb 2020

Keywords

Anoxybacillus sp.
detergents additive
lipase
oil-cake
response surface methodology
solid sate fermentation

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10.1080/10826068.2020.1719513

LPBB_2019_0317.R1_Accepted_Manuscript
This is an Accepted Manuscript of an article published by Taylor & Francis in Preparative Biochemistry & Biotechnology on 03/02/2020, available online: http://www.tandfonline.com/doi/full/10.1080/10826068.2020.171951.
Accepted author manuscript (Post-print), 1.9 MB

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title = "Parameter optimization for thermostable lipase production and performance evaluation as prospective detergent additive",

abstract = "Lipase based formulations has been a rising interest to laundry detergent industry for their ecofriendly property over phosphate-based counterparts and compatibility with chemical detergents ingredients. A thermo-stable Anoxybacillus sp. ARS-1 isolated from Taptapani Hotspring, India was characterized for optimum lipase production employing statistical model central composite design (CCD) under four independent variables (temperature, pH, % moisture and bio-surfactant) by solid substrate fermentation (SSF) using mustard cake. The output was utilized to find the effect of parameters and their interaction employing response surface methodology (RSM). A quadratic regression with R2¼0.955 established the model to be statically best fitting and a predicted highest lipase production of 29.4IU/g at an optimum temperature of 57.5oC, pH 8.31, moisture 50% and 1.2mg of bio-surfactant. Experimental production of 30.3IU/g lipase at above conditions validated the fitness of model. Anoxybacillus sp. ARS-1 produced lipase was found to resist almost all chemical detergents as well as common laundry detergent, proving it to be a prospective additive for incorporation.",

keywords = "Anoxybacillus sp., detergents additive, lipase, oil-cake, response surface methodology, solid sate fermentation",

author = "Sahoo, {Rajesh Kumar} and Aradhana Das and Mahendra Gaur and Anshuman Sahu and Saubhagini Sahoo and Suchanda Dey and Pattanathu Rahman and Enketeswara Subudhi",

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T1 - Parameter optimization for thermostable lipase production and performance evaluation as prospective detergent additive

AU - Sahoo, Rajesh Kumar

AU - Das, Aradhana

AU - Gaur, Mahendra

AU - Sahu, Anshuman

AU - Sahoo, Saubhagini

AU - Dey, Suchanda

AU - Rahman, Pattanathu

AU - Subudhi, Enketeswara

PY - 2020/2/3

Y1 - 2020/2/3

N2 - Lipase based formulations has been a rising interest to laundry detergent industry for their ecofriendly property over phosphate-based counterparts and compatibility with chemical detergents ingredients. A thermo-stable Anoxybacillus sp. ARS-1 isolated from Taptapani Hotspring, India was characterized for optimum lipase production employing statistical model central composite design (CCD) under four independent variables (temperature, pH, % moisture and bio-surfactant) by solid substrate fermentation (SSF) using mustard cake. The output was utilized to find the effect of parameters and their interaction employing response surface methodology (RSM). A quadratic regression with R2¼0.955 established the model to be statically best fitting and a predicted highest lipase production of 29.4IU/g at an optimum temperature of 57.5oC, pH 8.31, moisture 50% and 1.2mg of bio-surfactant. Experimental production of 30.3IU/g lipase at above conditions validated the fitness of model. Anoxybacillus sp. ARS-1 produced lipase was found to resist almost all chemical detergents as well as common laundry detergent, proving it to be a prospective additive for incorporation.

AB - Lipase based formulations has been a rising interest to laundry detergent industry for their ecofriendly property over phosphate-based counterparts and compatibility with chemical detergents ingredients. A thermo-stable Anoxybacillus sp. ARS-1 isolated from Taptapani Hotspring, India was characterized for optimum lipase production employing statistical model central composite design (CCD) under four independent variables (temperature, pH, % moisture and bio-surfactant) by solid substrate fermentation (SSF) using mustard cake. The output was utilized to find the effect of parameters and their interaction employing response surface methodology (RSM). A quadratic regression with R2¼0.955 established the model to be statically best fitting and a predicted highest lipase production of 29.4IU/g at an optimum temperature of 57.5oC, pH 8.31, moisture 50% and 1.2mg of bio-surfactant. Experimental production of 30.3IU/g lipase at above conditions validated the fitness of model. Anoxybacillus sp. ARS-1 produced lipase was found to resist almost all chemical detergents as well as common laundry detergent, proving it to be a prospective additive for incorporation.

KW - Anoxybacillus sp.

KW - detergents additive

KW - lipase

KW - oil-cake

KW - response surface methodology

KW - solid sate fermentation

U2 - 10.1080/10826068.2020.1719513

DO - 10.1080/10826068.2020.1719513

M3 - Article

SN - 1082-6068

JO - Preparative Biochemistry and Biotechnology

JF - Preparative Biochemistry and Biotechnology

ER -

Parameter optimization for thermostable lipase production and performance evaluation as prospective detergent additive

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Keywords

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