Parameter optimization for thermostable lipase production and performance evaluation as prospective detergent additive

Rajesh Kumar Sahoo, Aradhana Das, Mahendra Gaur, Anshuman Sahu, Saubhagini Sahoo, Suchanda Dey, Pattanathu Rahman, Enketeswara Subudhi

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Abstract

Lipase based formulations has been a rising interest to laundry detergent industry for their ecofriendly property over phosphate-based counterparts and compatibility with chemical detergents ingredients. A thermo-stable Anoxybacillus sp. ARS-1 isolated from Taptapani Hotspring, India was characterized for optimum lipase production employing statistical model central composite design (CCD) under four independent variables (temperature, pH, % moisture and bio-surfactant) by solid substrate fermentation (SSF) using mustard cake. The output was utilized to find the effect of parameters and their interaction employing response surface methodology (RSM). A quadratic regression with R2¼0.955 established the model to be statically best fitting and a predicted highest lipase production of 29.4IU/g at an optimum temperature of 57.5oC, pH 8.31, moisture 50% and 1.2mg of bio-surfactant. Experimental production of 30.3IU/g lipase at above conditions validated the fitness of model. Anoxybacillus sp. ARS-1 produced lipase was found to resist almost all chemical detergents as well as common laundry detergent, proving it to be a prospective additive for incorporation.
Original languageEnglish
Number of pages7
JournalPreparative Biochemistry and Biotechnology
Early online date3 Feb 2020
DOIs
Publication statusEarly online - 3 Feb 2020

Keywords

  • Anoxybacillus sp.
  • detergents additive
  • lipase
  • oil-cake
  • response surface methodology
  • solid sate fermentation

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