Performance tests of a novel coaxial tube catheter in an in vitro model of intracranial cell delivery

S. Panse; Helen Fillmore; Z. Chen; G. Gillies; W. Broaddus

doi:10.3109/03091902.2010.536296

Performance tests of a novel coaxial tube catheter in an in vitro model of intracranial cell delivery

S. Panse, Helen Fillmore, Z. Chen, G. Gillies, W. Broaddus

Research output: Contribution to journal › Article › peer-review

Abstract

We have tested prototypes of a novel coaxial tube catheter in an in vitro gel model of cell delivery into the brain. Devices 1.6 and 2.0 mm outer diameter were used to deliver PC 12 cells (concentration = 106 cells ml−1) at 1 μl min−1 into a 5 ml sandwich of collagen and 0.1% agarose, with and without follow-on infusions of nerve growth factor (NGF). Post-infusion microscopic imaging (40X) at the infusion sites was then carried out over 7-day periods. The results showed that under these experimental conditions, it was possible to use these catheters to deliver cells without either leakage of trapped air into the gel or reflux of the cell suspension along the catheter insertion track. Differentiation of the NGF-treated cells was observed.

Original language	English
Pages (from-to)	77-86
Number of pages	10
Journal	Journal of Medical Engineering & Technology
Volume	35
Issue number	2
DOIs	https://doi.org/10.3109/03091902.2010.536296
Publication status	Published - Feb 2011

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title = "Performance tests of a novel coaxial tube catheter in an in vitro model of intracranial cell delivery",

abstract = "We have tested prototypes of a novel coaxial tube catheter in an in vitro gel model of cell delivery into the brain. Devices 1.6 and 2.0 mm outer diameter were used to deliver PC 12 cells (concentration = 106 cells ml−1) at 1 μl min−1 into a 5 ml sandwich of collagen and 0.1% agarose, with and without follow-on infusions of nerve growth factor (NGF). Post-infusion microscopic imaging (40X) at the infusion sites was then carried out over 7-day periods. The results showed that under these experimental conditions, it was possible to use these catheters to deliver cells without either leakage of trapped air into the gel or reflux of the cell suspension along the catheter insertion track. Differentiation of the NGF-treated cells was observed.",

author = "S. Panse and Helen Fillmore and Z. Chen and G. Gillies and W. Broaddus",

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AU - Fillmore, Helen

AU - Chen, Z.

AU - Gillies, G.

AU - Broaddus, W.

PY - 2011/2

Y1 - 2011/2

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AB - We have tested prototypes of a novel coaxial tube catheter in an in vitro gel model of cell delivery into the brain. Devices 1.6 and 2.0 mm outer diameter were used to deliver PC 12 cells (concentration = 106 cells ml−1) at 1 μl min−1 into a 5 ml sandwich of collagen and 0.1% agarose, with and without follow-on infusions of nerve growth factor (NGF). Post-infusion microscopic imaging (40X) at the infusion sites was then carried out over 7-day periods. The results showed that under these experimental conditions, it was possible to use these catheters to deliver cells without either leakage of trapped air into the gel or reflux of the cell suspension along the catheter insertion track. Differentiation of the NGF-treated cells was observed.

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Performance tests of a novel coaxial tube catheter in an in vitro model of intracranial cell delivery

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