TY - JOUR
T1 - Plasminogen binding proteins in secreted membrane vesicles of Leishmania mexicana
AU - Figuera, Lourdes
AU - Acosta, Héctor
AU - Gómez-Arreaza, Amaranta
AU - Dávila-Vera, Delsy
AU - Balza-Quintero, Alirio
AU - Quiñones, Wilfredo
AU - Mendoza-Briceño, Rosa Virginia
AU - Concepción, Juan Luis
AU - Avilán, Luisana
N1 - Copyright © 2012 Elsevier B.V. All rights reserved.
PY - 2013/1
Y1 - 2013/1
N2 - Membrane vesicles secreted by Leishmania mexicana were collected and analyzed. These vesicles can bind plasminogen and were shown to contain enolase, previously identified as a plasminogen-binding protein. In addition, another plasminogen-binding protein was identified, the small myristoylated protein, SMP-1. Recombinant SMP-1 was able to bind plasminogen in a lysine-dependent manner with a K(d) value of 0.24 μM. The C-terminal lysine seems to be responsible for this binding, since this recognition decreases upon carboxypeptidase B treatment. This protein was present within the secreted membrane vesicles as demonstrated by its protection from trypsin digestion in the absence of Triton X-100. Plasminogen-binding proteins in the secreted vesicles may be involved in parasite invasion in the mammalian host.
AB - Membrane vesicles secreted by Leishmania mexicana were collected and analyzed. These vesicles can bind plasminogen and were shown to contain enolase, previously identified as a plasminogen-binding protein. In addition, another plasminogen-binding protein was identified, the small myristoylated protein, SMP-1. Recombinant SMP-1 was able to bind plasminogen in a lysine-dependent manner with a K(d) value of 0.24 μM. The C-terminal lysine seems to be responsible for this binding, since this recognition decreases upon carboxypeptidase B treatment. This protein was present within the secreted membrane vesicles as demonstrated by its protection from trypsin digestion in the absence of Triton X-100. Plasminogen-binding proteins in the secreted vesicles may be involved in parasite invasion in the mammalian host.
KW - Host-Pathogen Interactions
KW - Kinetics
KW - Leishmania mexicana/metabolism
KW - Lysine/metabolism
KW - Plasminogen/metabolism
KW - Protein Binding
KW - Protozoan Proteins/metabolism
KW - Secretory Vesicles/metabolism
U2 - 10.1016/j.molbiopara.2012.11.002
DO - 10.1016/j.molbiopara.2012.11.002
M3 - Article
C2 - 23178693
SN - 0166-6851
VL - 187
SP - 14
EP - 20
JO - Molecular and Biochemical Parasitology
JF - Molecular and Biochemical Parasitology
IS - 1
ER -