Plasminogen binding proteins in secreted membrane vesicles of Leishmania mexicana

Lourdes Figuera; Héctor Acosta; Amaranta Gómez-Arreaza; Delsy Dávila-Vera; Alirio Balza-Quintero; Wilfredo Quiñones; Rosa Virginia Mendoza-Briceño; Juan Luis Concepción; Luisana Avilán

doi:10.1016/j.molbiopara.2012.11.002

Plasminogen binding proteins in secreted membrane vesicles of Leishmania mexicana

Lourdes Figuera, Héctor Acosta, Amaranta Gómez-Arreaza, Delsy Dávila-Vera, Alirio Balza-Quintero, Wilfredo Quiñones, Rosa Virginia Mendoza-Briceño, Juan Luis Concepción, Luisana Avilán

School of Biological Sciences

Universidad de los Andes Mérida

Research output: Contribution to journal › Article › peer-review

Abstract

Membrane vesicles secreted by Leishmania mexicana were collected and analyzed. These vesicles can bind plasminogen and were shown to contain enolase, previously identified as a plasminogen-binding protein. In addition, another plasminogen-binding protein was identified, the small myristoylated protein, SMP-1. Recombinant SMP-1 was able to bind plasminogen in a lysine-dependent manner with a K(d) value of 0.24 μM. The C-terminal lysine seems to be responsible for this binding, since this recognition decreases upon carboxypeptidase B treatment. This protein was present within the secreted membrane vesicles as demonstrated by its protection from trypsin digestion in the absence of Triton X-100. Plasminogen-binding proteins in the secreted vesicles may be involved in parasite invasion in the mammalian host.

Original language	English
Pages (from-to)	14-20
Number of pages	7
Journal	Molecular and Biochemical Parasitology
Volume	187
Issue number	1
Early online date	22 Nov 2012
DOIs	https://doi.org/10.1016/j.molbiopara.2012.11.002
Publication status	Published - Jan 2013

Keywords

Host-Pathogen Interactions
Kinetics
Leishmania mexicana/metabolism
Lysine/metabolism
Plasminogen/metabolism
Protein Binding
Protozoan Proteins/metabolism
Secretory Vesicles/metabolism

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10.1016/j.molbiopara.2012.11.002

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title = "Plasminogen binding proteins in secreted membrane vesicles of Leishmania mexicana",

abstract = "Membrane vesicles secreted by Leishmania mexicana were collected and analyzed. These vesicles can bind plasminogen and were shown to contain enolase, previously identified as a plasminogen-binding protein. In addition, another plasminogen-binding protein was identified, the small myristoylated protein, SMP-1. Recombinant SMP-1 was able to bind plasminogen in a lysine-dependent manner with a K(d) value of 0.24 μM. The C-terminal lysine seems to be responsible for this binding, since this recognition decreases upon carboxypeptidase B treatment. This protein was present within the secreted membrane vesicles as demonstrated by its protection from trypsin digestion in the absence of Triton X-100. Plasminogen-binding proteins in the secreted vesicles may be involved in parasite invasion in the mammalian host.",

keywords = "Host-Pathogen Interactions, Kinetics, Leishmania mexicana/metabolism, Lysine/metabolism, Plasminogen/metabolism, Protein Binding, Protozoan Proteins/metabolism, Secretory Vesicles/metabolism",

author = "Lourdes Figuera and H{\'e}ctor Acosta and Amaranta G{\'o}mez-Arreaza and Delsy D{\'a}vila-Vera and Alirio Balza-Quintero and Wilfredo Qui{\~n}ones and Mendoza-Brice{\~n}o, {Rosa Virginia} and Concepci{\'o}n, {Juan Luis} and Luisana Avil{\'a}n",

year = "2013",

month = jan,

doi = "10.1016/j.molbiopara.2012.11.002",

language = "English",

volume = "187",

pages = "14--20",

journal = "Molecular and Biochemical Parasitology",

issn = "0166-6851",

publisher = "Elsevier",

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TY - JOUR

T1 - Plasminogen binding proteins in secreted membrane vesicles of Leishmania mexicana

AU - Figuera, Lourdes

AU - Acosta, Héctor

AU - Gómez-Arreaza, Amaranta

AU - Dávila-Vera, Delsy

AU - Balza-Quintero, Alirio

AU - Quiñones, Wilfredo

AU - Mendoza-Briceño, Rosa Virginia

AU - Concepción, Juan Luis

AU - Avilán, Luisana

PY - 2013/1

Y1 - 2013/1

N2 - Membrane vesicles secreted by Leishmania mexicana were collected and analyzed. These vesicles can bind plasminogen and were shown to contain enolase, previously identified as a plasminogen-binding protein. In addition, another plasminogen-binding protein was identified, the small myristoylated protein, SMP-1. Recombinant SMP-1 was able to bind plasminogen in a lysine-dependent manner with a K(d) value of 0.24 μM. The C-terminal lysine seems to be responsible for this binding, since this recognition decreases upon carboxypeptidase B treatment. This protein was present within the secreted membrane vesicles as demonstrated by its protection from trypsin digestion in the absence of Triton X-100. Plasminogen-binding proteins in the secreted vesicles may be involved in parasite invasion in the mammalian host.

AB - Membrane vesicles secreted by Leishmania mexicana were collected and analyzed. These vesicles can bind plasminogen and were shown to contain enolase, previously identified as a plasminogen-binding protein. In addition, another plasminogen-binding protein was identified, the small myristoylated protein, SMP-1. Recombinant SMP-1 was able to bind plasminogen in a lysine-dependent manner with a K(d) value of 0.24 μM. The C-terminal lysine seems to be responsible for this binding, since this recognition decreases upon carboxypeptidase B treatment. This protein was present within the secreted membrane vesicles as demonstrated by its protection from trypsin digestion in the absence of Triton X-100. Plasminogen-binding proteins in the secreted vesicles may be involved in parasite invasion in the mammalian host.

KW - Host-Pathogen Interactions

KW - Kinetics

KW - Leishmania mexicana/metabolism

KW - Lysine/metabolism

KW - Plasminogen/metabolism

KW - Protein Binding

KW - Protozoan Proteins/metabolism

KW - Secretory Vesicles/metabolism

U2 - 10.1016/j.molbiopara.2012.11.002

DO - 10.1016/j.molbiopara.2012.11.002

M3 - Article

C2 - 23178693

VL - 187

SP - 14

EP - 20

JO - Molecular and Biochemical Parasitology

JF - Molecular and Biochemical Parasitology

SN - 0166-6851

IS - 1

ER -

Plasminogen binding proteins in secreted membrane vesicles of Leishmania mexicana

Abstract

Keywords

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