Plasminogen binding proteins in secreted membrane vesicles of Leishmania mexicana

Lourdes Figuera, Héctor Acosta, Amaranta Gómez-Arreaza, Delsy Dávila-Vera, Alirio Balza-Quintero, Wilfredo Quiñones, Rosa Virginia Mendoza-Briceño, Juan Luis Concepción, Luisana Avilán

Research output: Contribution to journalArticlepeer-review

Abstract

Membrane vesicles secreted by Leishmania mexicana were collected and analyzed. These vesicles can bind plasminogen and were shown to contain enolase, previously identified as a plasminogen-binding protein. In addition, another plasminogen-binding protein was identified, the small myristoylated protein, SMP-1. Recombinant SMP-1 was able to bind plasminogen in a lysine-dependent manner with a K(d) value of 0.24 μM. The C-terminal lysine seems to be responsible for this binding, since this recognition decreases upon carboxypeptidase B treatment. This protein was present within the secreted membrane vesicles as demonstrated by its protection from trypsin digestion in the absence of Triton X-100. Plasminogen-binding proteins in the secreted vesicles may be involved in parasite invasion in the mammalian host.

Original languageEnglish
Pages (from-to)14-20
Number of pages7
JournalMolecular and Biochemical Parasitology
Volume187
Issue number1
Early online date22 Nov 2012
DOIs
Publication statusPublished - Jan 2013

Keywords

  • Host-Pathogen Interactions
  • Kinetics
  • Leishmania mexicana/metabolism
  • Lysine/metabolism
  • Plasminogen/metabolism
  • Protein Binding
  • Protozoan Proteins/metabolism
  • Secretory Vesicles/metabolism

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