Preparation and characterization of bioadhesive microparticles comprised of low degree of quaternization trimethylated chitosan for nasal administration: effect of concentration and molecular weigh

Christina Karavasili, Orestis Katsamenis, Nikolaos Bouropoulos, Hamde Nazar, Philipp Thurner, Marisa Van Der Merwe, Dimitrios Fatouros

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    Abstract

    Toward the development of microparticulate carriers for nasal administration, N-trimethylchitosan chloride (TMC) of low molecular weight (LMW) and high molecular weight (HMW) and low degree of quaternization (16% and 27%, respectively) was co-formulated into microparticles comprising of dipalmatoylphosphatidylcholine (DPPC) and poly(lactic-co-glycolic) acid (PLGA) via the spray-drying technique. The chitosan derivatives were characterized by means of nuclear magnetic resonance (NMR), differential scanning calorimetry (DSC), and Fourier transfrom infrared (FTIR) spectroscopy. The size and morphology of the produced microparticles were assessed by scanning electron microscopy (SEM), whereas their mucoadhesive properties were investigated by means of atomic force microscopy–force spectroscopy (AFM-FS). The results showed that microparticles exhibit mucoadhesion when TMC is present on their surface above a threshold of TMC (>0.3% w/w).
    Original languageEnglish
    Pages (from-to)12337–12344
    JournalLangmuir
    Volume30
    Issue number41
    Early online date23 Sept 2014
    DOIs
    Publication statusPublished - 7 Oct 2014

    Keywords

    • Nasal delivery, chitosan derivatives, mucoadhesion, Atomic force microscopy

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