Probing for chemotherapy-induced peripheral neuropathy in live dorsal root ganglion neurons with atomic force microscopy

Ngan Pan Bennett Au, Yuqiang Fang, Ning Xi, King Wai Chiu Lai, Chi Him Eddie Ma

Research output: Contribution to journalArticlepeer-review


Chemotherapy-induced peripheral neuropathy (CIPN) remains a major reason for cancer patients to withdraw from their lifesaving therapy. CIPN results in irreversible sensory and motor impairments; however, the epidemiology is largely unknown. Here, we report for the first time that chemotherapy drug vincristine not only reduced axonal regeneration in primary dorsal root ganglion neuron but also induced substantial changes in cell mechanical properties detected by atomic force microscopy (AFM). Confocal imaging analysis revealed vincristine-induced microtubule depolymerization. By using AFM for high-resolution live cell imaging and quantitative analysis, we observed significant changes in cell surface roughness and stiffness of vincristine-treated neurons. Elastic modulus was decreased (21-45%) with increasing dosage of vincristine. Further study with paclitaxel, another well-known CIPN drug, confirmed the link between cell mechanics and cytoskeleton organization. These data support that our system can be used for probing potential CIPN drugs that are of enormous benefit to new chemotherapy drug development. From the Clinical Editor: This study concludes that reduced cell elasticity in dorsal root ganglion neurons accompanies the development of chemotherapy-induced peripheral neuropathy, providing a model system that enables testing of upcoming chemotherapy agents for this particularly inconvenient and often treatment-limiting complication.

Original languageEnglish
Pages (from-to)1323-1333
Number of pages11
JournalNanomedicine: Nanotechnology, Biology, and Medicine
Issue number6
Early online date12 Mar 2014
Publication statusPublished - 1 Aug 2014


  • AFM live cell imaging
  • cell mechanic
  • chemotherapy-induced peripheral neuropathy
  • dorsal root ganglion neurons
  • nanoindentation

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