Production of rhamnolipid biosurfactants by Pseudomonas aeruginosa DS10-129 in a microfluidic bioreactor

Pattanathu K. S. M. Rahman*, Godfrey Pasirayi, Vincent Auger, Zulfiqur Ali

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review


A low-cost μBR (microbioreactor) made from PTFE [poly(tetrafluoroethylene)] was used to cultivate a model organism, Pseudomonas aeruginosa DS10-129. The progress of bioprocessing was monitored by comparing the growth of the organism in a μBR, a conventional bench scale bioreactor and a shake flask. Under the μBR conditions, the organism produced 23 mg/ml of pyocyanin that had antimicrobial effects against Bacillus subtilis, Staphylococcus aureus, Staphylococcus epidermidis, Pseudomonas teessidea and Pseudomonas clemancea. Furthermore, it produced a total of 106 μg/ml of effective biosurfactants consisting of dirhamnolipids (RL2) and monorhamnolipids (RL1). The biosurfactants reduced the surface tension of distilled water from 72 to 27.9 mN/m and emulsified kerosene by 71.30%. The pyocyanin and rhamnolipids were produced during the exponential and stationary phases of growth respectively. The results of the μBR were comparable to those obtained using conventional scale methods.

Original languageEnglish
Pages (from-to)45-52
Number of pages8
JournalBiotechnology and Applied Biochemistry
Issue number1
Publication statusPublished - 23 Dec 2010


  • Antimicrobial activity
  • Biosurfactant
  • High-throughput bioprocessing
  • Microbioreactor (μBR)
  • Microfluidic bioreactor
  • Rhamnolipid


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