Radiation damage to nucleoprotein complexes in macromolecular crystallography

Charles Bury, Elspeth F. Garman, Helen Mary Ginn, Raimond B. G. Ravelli, Ian Carmichael, Geoff Kneale, John E. Mcgeehan

Research output: Contribution to journalArticlepeer-review

323 Downloads (Pure)

Abstract

Significant progress has been made in macromolecular crystallography over recent years in both the understanding and mitigation of X-ray induced radiation damage when collecting diffraction data from crystalline proteins. In contrast, despite the large field that is productively engaged in the study of radiation chemistry of nucleic acids, particularly of DNA, there are currently very few X-ray crystallographic studies on radiation damage mechanisms in nucleic acids. Quantitative comparison of damage to protein and DNA crystals separately is challenging, but many of the issues are circumvented by studying pre-formed biological nucleoprotein complexes where direct comparison of each component can be made under the same controlled conditions. Here a model protein-DNA complex C.Esp1396I is employed to investigate specific damage mechanisms for protein and DNA in a biologically relevant complex over a large dose range (2.07-44.63 MGy). In order to allow a quantitative analysis of radiation damage sites from a complex series of macromolecular diffraction data, a computational method has been developed that is generally applicable to the field. Typical specific damage was observed for both the protein on particular amino acids and for the DNA on, for example, the cleavage of base-sugar N1-C and sugar-phosphate C-O bonds. Strikingly the DNA component was determined to be far more resistant to specific damage than the protein for the investigated dose range. At low doses the protein was observed to be susceptible to radiation damage while the DNA was far more resistant, damage only being observed at significantly higher doses.
Original languageEnglish
Pages (from-to)213-224
JournalJournal of Synchrotron Radiation
Volume22
Early online date30 Jan 2015
DOIs
Publication statusPublished - 1 Mar 2015

Keywords

  • RCUK
  • BBSRC
  • BB/E000878/1

Fingerprint

Dive into the research topics of 'Radiation damage to nucleoprotein complexes in macromolecular crystallography'. Together they form a unique fingerprint.

Cite this