Random mutagenesis by PCR

    Research output: Chapter in Book/Report/Conference proceedingChapter (peer-reviewed)peer-review

    Abstract

    Random mutagenesis of DNA sequences has the advantage of generating DNA sequences with novel properties, either directly in the case of aptamers or through subsequent transcription/translation of the mutated sequence in the case of proteins. In both cases no prior structural or mechanistic knowledge of the molecule is required. For sequences greater than 100 bp, one of the easiest methods to introduce the mutations is to use Error-prone PCR (EP-PCR) as discussed in this chapter. When coupled with an appropriate selection or high throughput screening methodology, PCR-based random mutagenesis can provide a powerful tool for modern molecular biologists.
    Original languageEnglish
    Title of host publicationDNA Manipulation and Analysis
    EditorsGarry Scarlett
    Place of PublicationNew York
    PublisherHumana Press
    Chapter7
    Pages81-86
    Number of pages6
    Edition1st
    ISBN (Electronic)9781071630044
    ISBN (Print)9781071630037
    DOIs
    Publication statusPublished - 1 Mar 2023

    Publication series

    NameMethods in Molecular Biology
    Volume2633
    ISSN (Print)1064-3745
    ISSN (Electronic)1940-6029

    Keywords

    • random mutagenesis
    • PCR
    • error-prone

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