Recombinant hTASK1 is an O2-sensitive K+ channel

Anthony Lewis, M. Hartness, C. Chapman, I. Fearon, H. Meadows, C. Peers, P. Kemp

Research output: Contribution to journalArticlepeer-review


Hypoxic inhibition of background K+ channels is crucial to O2 sensing by chemoreceptor tissues, but direct demonstration of O2 sensitivity by any member of this K+ channel family is lacking. HEK293 cells were transfected with a pcDNA3.1-hTASK1 construct; expression of hTASK1 was verified using RT-PCR and immunocytochemistry. Whole-cell K+ currents of cells stably expressing hTASK-1 were, as anticipated, extremely sensitive to extracellular pH, within the physiological range (IC50 ≈ 7.0). All cells expressing this signature pH sensitivity were acutely modulated by pO2; reduction of pO2 from 150 to
Original languageEnglish
Pages (from-to)1290-1294
Number of pages5
JournalBiochemical and Biophysical Research Communications
Issue number5
Publication statusPublished - 2001


Dive into the research topics of 'Recombinant hTASK1 is an O2-sensitive K+ channel'. Together they form a unique fingerprint.

Cite this