Abstract
Hypoxic inhibition of background K+ channels is crucial to O2 sensing by chemoreceptor tissues, but direct demonstration of O2 sensitivity by any member of this K+ channel family is lacking. HEK293 cells were transfected with a pcDNA3.1-hTASK1 construct; expression of hTASK1 was verified using RT-PCR and immunocytochemistry. Whole-cell K+ currents of cells stably expressing hTASK-1 were, as anticipated, extremely sensitive to extracellular pH, within the physiological range (IC50 ≈ 7.0). All cells expressing this signature pH sensitivity were acutely modulated by pO2; reduction of pO2 from 150 to
Original language | English |
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Pages (from-to) | 1290-1294 |
Number of pages | 5 |
Journal | Biochemical and Biophysical Research Communications |
Volume | 285 |
Issue number | 5 |
DOIs | |
Publication status | Published - 2001 |