Abstract
Background: Immature platelet fraction (IPF) estimation is a non-invasive and sensitive test that is available on recently introduced Sysmex XN-series of automated haematology analysers. It is a direct cellular indicator of thrombopoiesis. The aim of this study was to establish reference intervals for IPF, for both absolute (A-IPF) and percentage (%-IPF) measurements.
Material and methods: A total of 2366 samples that met the inclusion criteria were assayed for full blood count on the Sysmex XN-10 and a non-parametric percentile method was used for calculating the reference intervals.
Results: After the outliers were excluded, the reference interval for %-IPF and A-IPF on Sysmex XN-10 were 1.6–10.1% and 4.37–23.21 × 109/L in total individuals, respectively. There was a statistical significance noted between the sexes (p = .004) for %-IPF, therefore a sex-specific reference interval was established, which was 1.8–10.0% for the males and 1.5–10.1% for females. No significant difference in sex status for A-IPF and age status for both %-IPF and A-IPF was observed. A very poor correlation was estimated between age versus %-IPF, ρ = 0.0156, and age versus A-IPF, ρ = −0.0023, indicating that there is no overall biological relationship between age and these parameters. As expected, a strong correlation between %-IPF and A-IPF was noted which could be attributed to their inter-relatedness.
Conclusions: This large-scale study showed comparable reference intervals with the previous studies for %-IPF and A-IPF in a UK population. It found the need to establish sex-specific reference intervals for %-IPF, but not for A-IPF, whereas reference intervals were found to be stable across the age range.
Material and methods: A total of 2366 samples that met the inclusion criteria were assayed for full blood count on the Sysmex XN-10 and a non-parametric percentile method was used for calculating the reference intervals.
Results: After the outliers were excluded, the reference interval for %-IPF and A-IPF on Sysmex XN-10 were 1.6–10.1% and 4.37–23.21 × 109/L in total individuals, respectively. There was a statistical significance noted between the sexes (p = .004) for %-IPF, therefore a sex-specific reference interval was established, which was 1.8–10.0% for the males and 1.5–10.1% for females. No significant difference in sex status for A-IPF and age status for both %-IPF and A-IPF was observed. A very poor correlation was estimated between age versus %-IPF, ρ = 0.0156, and age versus A-IPF, ρ = −0.0023, indicating that there is no overall biological relationship between age and these parameters. As expected, a strong correlation between %-IPF and A-IPF was noted which could be attributed to their inter-relatedness.
Conclusions: This large-scale study showed comparable reference intervals with the previous studies for %-IPF and A-IPF in a UK population. It found the need to establish sex-specific reference intervals for %-IPF, but not for A-IPF, whereas reference intervals were found to be stable across the age range.
| Original language | English |
|---|---|
| Pages (from-to) | 658-664 |
| Number of pages | 7 |
| Journal | Scandinavian Journal of Clinical and Laboratory Investigation |
| Volume | 77 |
| Issue number | 8 |
| Early online date | 9 Nov 2017 |
| DOIs | |
| Publication status | Published - 1 Dec 2017 |
Keywords
- reference values
- blood platelet
- haematology
- thrombocytopenia
- blood cell count
- haematologic tests
- haematologic diseases
- clinicla laboratory techniques
- clinical laboratory services
- UK