Regulated expression of Brachyury(T), Nkx1.1 and Pax genes in embryoid bodies

G. Yamada, C. Kioussi, Frank R. Schubert, Y. Eto, K. Chowdhury, F. Pituello, P. Gruss

Research output: Contribution to journalArticlepeer-review


Embryonic stem cells (ES) can be exploited to analyze in vitro mechanisms of cellular differentiation. We have utilized ES-derived embryoid body formation in an attempt to study cell types resulting from in vitro differentiation. To this end, a variety of molecular markers, preferably those which have been associated with regulatory events during mouse embryogenesis, was employed. Specifically, Brachyury (T), Pax-3 and Pax-6 genes as well as Nkx-1.1 were used. We could demonstrate that the expression of these genes in vitro was regulated by growth factors such as activin A or bFGF. Implications of these findings and the possible applications for identifying new genes are discussed.

Original languageEnglish
Pages (from-to)552-63
JournalBiochemical and Biophysical Research Communications
Issue number2
Publication statusPublished - 15 Mar 1994


  • Animals
  • Cells, Cultured
  • DNA-Binding Proteins
  • Embryo, Nonmammalian
  • Embryonic and Fetal Development
  • Eye Proteins
  • Fetal Proteins
  • Gene Expression Regulation
  • Genes, Homeobox
  • Glial Fibrillary Acidic Protein
  • Homeodomain Proteins
  • In Situ Hybridization, Fluorescence
  • Mesoderm
  • Nerve Growth Factors
  • Neurons
  • Paired Box Transcription Factors
  • Repressor Proteins
  • Stem Cells
  • T-Box Domain Proteins
  • Transcription Factors
  • Xenopus
  • Xenopus Proteins


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