Abstract
Embryonic stem cells (ES) can be exploited to analyze in vitro mechanisms of cellular differentiation. We have utilized ES-derived embryoid body formation in an attempt to study cell types resulting from in vitro differentiation. To this end, a variety of molecular markers, preferably those which have been associated with regulatory events during mouse embryogenesis, was employed. Specifically, Brachyury (T), Pax-3 and Pax-6 genes as well as Nkx-1.1 were used. We could demonstrate that the expression of these genes in vitro was regulated by growth factors such as activin A or bFGF. Implications of these findings and the possible applications for identifying new genes are discussed.
Original language | English |
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Pages (from-to) | 552-63 |
Journal | Biochemical and Biophysical Research Communications |
Volume | 199 |
Issue number | 2 |
DOIs | |
Publication status | Published - 15 Mar 1994 |
Keywords
- Animals
- Cells, Cultured
- DNA-Binding Proteins
- Embryo, Nonmammalian
- Embryonic and Fetal Development
- Eye Proteins
- Fetal Proteins
- Gene Expression Regulation
- Genes, Homeobox
- Glial Fibrillary Acidic Protein
- Homeodomain Proteins
- In Situ Hybridization, Fluorescence
- Mesoderm
- Nerve Growth Factors
- Neurons
- Paired Box Transcription Factors
- Repressor Proteins
- Stem Cells
- T-Box Domain Proteins
- Transcription Factors
- Xenopus
- Xenopus Proteins