Regulated expression of Brachyury(T), Nkx1.1 and Pax genes in embryoid bodies

G. Yamada; C. Kioussi; Frank R. Schubert; Y. Eto; K. Chowdhury; F. Pituello; P. Gruss

doi:10.1006/bbrc.1994.1264

Regulated expression of Brachyury(T), Nkx1.1 and Pax genes in embryoid bodies

G. Yamada, C. Kioussi, Frank R. Schubert, Y. Eto, K. Chowdhury, F. Pituello, P. Gruss

Max Planck Institute of Biophysical Chemistry

Research output: Contribution to journal › Article › peer-review

Abstract

Embryonic stem cells (ES) can be exploited to analyze in vitro mechanisms of cellular differentiation. We have utilized ES-derived embryoid body formation in an attempt to study cell types resulting from in vitro differentiation. To this end, a variety of molecular markers, preferably those which have been associated with regulatory events during mouse embryogenesis, was employed. Specifically, Brachyury (T), Pax-3 and Pax-6 genes as well as Nkx-1.1 were used. We could demonstrate that the expression of these genes in vitro was regulated by growth factors such as activin A or bFGF. Implications of these findings and the possible applications for identifying new genes are discussed.

Original language	English
Pages (from-to)	552-63
Journal	Biochemical and Biophysical Research Communications
Volume	199
Issue number	2
DOIs	https://doi.org/10.1006/bbrc.1994.1264
Publication status	Published - 15 Mar 1994

Keywords

Animals
Cells, Cultured
DNA-Binding Proteins
Embryo, Nonmammalian
Embryonic and Fetal Development
Eye Proteins
Fetal Proteins
Gene Expression Regulation
Genes, Homeobox
Glial Fibrillary Acidic Protein
Homeodomain Proteins
In Situ Hybridization, Fluorescence
Mesoderm
Nerve Growth Factors
Neurons
Paired Box Transcription Factors
Repressor Proteins
Stem Cells
T-Box Domain Proteins
Transcription Factors
Xenopus
Xenopus Proteins

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title = "Regulated expression of Brachyury(T), Nkx1.1 and Pax genes in embryoid bodies",

abstract = "Embryonic stem cells (ES) can be exploited to analyze in vitro mechanisms of cellular differentiation. We have utilized ES-derived embryoid body formation in an attempt to study cell types resulting from in vitro differentiation. To this end, a variety of molecular markers, preferably those which have been associated with regulatory events during mouse embryogenesis, was employed. Specifically, Brachyury (T), Pax-3 and Pax-6 genes as well as Nkx-1.1 were used. We could demonstrate that the expression of these genes in vitro was regulated by growth factors such as activin A or bFGF. Implications of these findings and the possible applications for identifying new genes are discussed.",

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author = "G. Yamada and C. Kioussi and Schubert, \{Frank R.\} and Y. Eto and K. Chowdhury and F. Pituello and P. Gruss",

year = "1994",

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doi = "10.1006/bbrc.1994.1264",

language = "English",

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journal = "Biochemical and Biophysical Research Communications",

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T1 - Regulated expression of Brachyury(T), Nkx1.1 and Pax genes in embryoid bodies

AU - Yamada, G.

AU - Kioussi, C.

AU - Schubert, Frank R.

AU - Eto, Y.

AU - Chowdhury, K.

AU - Pituello, F.

AU - Gruss, P.

PY - 1994/3/15

Y1 - 1994/3/15

N2 - Embryonic stem cells (ES) can be exploited to analyze in vitro mechanisms of cellular differentiation. We have utilized ES-derived embryoid body formation in an attempt to study cell types resulting from in vitro differentiation. To this end, a variety of molecular markers, preferably those which have been associated with regulatory events during mouse embryogenesis, was employed. Specifically, Brachyury (T), Pax-3 and Pax-6 genes as well as Nkx-1.1 were used. We could demonstrate that the expression of these genes in vitro was regulated by growth factors such as activin A or bFGF. Implications of these findings and the possible applications for identifying new genes are discussed.

AB - Embryonic stem cells (ES) can be exploited to analyze in vitro mechanisms of cellular differentiation. We have utilized ES-derived embryoid body formation in an attempt to study cell types resulting from in vitro differentiation. To this end, a variety of molecular markers, preferably those which have been associated with regulatory events during mouse embryogenesis, was employed. Specifically, Brachyury (T), Pax-3 and Pax-6 genes as well as Nkx-1.1 were used. We could demonstrate that the expression of these genes in vitro was regulated by growth factors such as activin A or bFGF. Implications of these findings and the possible applications for identifying new genes are discussed.

KW - Animals

KW - Cells, Cultured

KW - DNA-Binding Proteins

KW - Embryo, Nonmammalian

KW - Embryonic and Fetal Development

KW - Eye Proteins

KW - Fetal Proteins

KW - Gene Expression Regulation

KW - Genes, Homeobox

KW - Glial Fibrillary Acidic Protein

KW - Homeodomain Proteins

KW - In Situ Hybridization, Fluorescence

KW - Mesoderm

KW - Nerve Growth Factors

KW - Neurons

KW - Paired Box Transcription Factors

KW - Repressor Proteins

KW - Stem Cells

KW - T-Box Domain Proteins

KW - Transcription Factors

KW - Xenopus

KW - Xenopus Proteins

U2 - 10.1006/bbrc.1994.1264

DO - 10.1006/bbrc.1994.1264

M3 - Article

C2 - 7907867

SN - 0006-291X

VL - 199

SP - 552

EP - 563

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

IS - 2

ER -

Regulated expression of Brachyury(T), Nkx1.1 and Pax genes in embryoid bodies

Abstract

Keywords

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