Regulation and induction of CYP3A11, CYP3A13 and CYP3A25 in C57BL/6J mouse liver

M. J. Down; S. Arkle; J. J. Mills

doi:10.1016/j.abb.2006.09.017

Regulation and induction of CYP3A11, CYP3A13 and CYP3A25 in C57BL/6J mouse liver

M. J. Down, S. Arkle, J. J. Mills^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

Abstract

This study reports that dexamethasone (DEX) significantly induces CYP3A11, CYP3A13 and CYP3A25 mRNA expression in male and female 4 days, 3 weeks and 18 weeks old C57BL/6J mice. Furthermore, CYP3A activity, as measured by erythromycin-N-demethylation, is also significantly increased. PXR, RXRα and CAR are known to be involved in the induction of CYP3As. Here we report nuclear receptors PXR and RXRα but not CAR demonstrate gender- and age-dependent expression. Also, treatment of C57BL/6J mice with DEX induces PXR but not RXRα or CAR. In summary, we demonstrate DEX is not only able to up-regulate CYP3A expression and activity, but also the nuclear receptor PXR through which it may exert this effect. Furthermore, the gender- and age-dependent pattern of basal PXR and RXRα expression is similar to the 3 CYP3As analysed.

Original language	English
Pages (from-to)	105-110
Number of pages	6
Journal	Archives of Biochemistry and Biophysics
Volume	457
Issue number	1
DOIs	https://doi.org/10.1016/j.abb.2006.09.017
Publication status	Published - 1 Jan 2007

Keywords

CAR
CYP3A11
CYP3A13
CYP3A25
DEX
Expression
Induction
Mouse
PXR
RXRα

Access to Document

10.1016/j.abb.2006.09.017

Cite this

@article{c61bf7bd7e9b4f01b6516e65ce358885,

title = "Regulation and induction of CYP3A11, CYP3A13 and CYP3A25 in C57BL/6J mouse liver",

abstract = "This study reports that dexamethasone (DEX) significantly induces CYP3A11, CYP3A13 and CYP3A25 mRNA expression in male and female 4 days, 3 weeks and 18 weeks old C57BL/6J mice. Furthermore, CYP3A activity, as measured by erythromycin-N-demethylation, is also significantly increased. PXR, RXRα and CAR are known to be involved in the induction of CYP3As. Here we report nuclear receptors PXR and RXRα but not CAR demonstrate gender- and age-dependent expression. Also, treatment of C57BL/6J mice with DEX induces PXR but not RXRα or CAR. In summary, we demonstrate DEX is not only able to up-regulate CYP3A expression and activity, but also the nuclear receptor PXR through which it may exert this effect. Furthermore, the gender- and age-dependent pattern of basal PXR and RXRα expression is similar to the 3 CYP3As analysed.",

keywords = "CAR, CYP3A11, CYP3A13, CYP3A25, DEX, Expression, Induction, Mouse, PXR, RXRα",

author = "Down, {M. J.} and S. Arkle and Mills, {J. J.}",

year = "2007",

month = jan,

day = "1",

doi = "10.1016/j.abb.2006.09.017",

language = "English",

volume = "457",

pages = "105--110",

journal = "Archives of Biochemistry and Biophysics",

issn = "0003-9861",

publisher = "Academic Press Inc.",

number = "1",

}

TY - JOUR

T1 - Regulation and induction of CYP3A11, CYP3A13 and CYP3A25 in C57BL/6J mouse liver

AU - Down, M. J.

AU - Arkle, S.

AU - Mills, J. J.

PY - 2007/1/1

Y1 - 2007/1/1

N2 - This study reports that dexamethasone (DEX) significantly induces CYP3A11, CYP3A13 and CYP3A25 mRNA expression in male and female 4 days, 3 weeks and 18 weeks old C57BL/6J mice. Furthermore, CYP3A activity, as measured by erythromycin-N-demethylation, is also significantly increased. PXR, RXRα and CAR are known to be involved in the induction of CYP3As. Here we report nuclear receptors PXR and RXRα but not CAR demonstrate gender- and age-dependent expression. Also, treatment of C57BL/6J mice with DEX induces PXR but not RXRα or CAR. In summary, we demonstrate DEX is not only able to up-regulate CYP3A expression and activity, but also the nuclear receptor PXR through which it may exert this effect. Furthermore, the gender- and age-dependent pattern of basal PXR and RXRα expression is similar to the 3 CYP3As analysed.

AB - This study reports that dexamethasone (DEX) significantly induces CYP3A11, CYP3A13 and CYP3A25 mRNA expression in male and female 4 days, 3 weeks and 18 weeks old C57BL/6J mice. Furthermore, CYP3A activity, as measured by erythromycin-N-demethylation, is also significantly increased. PXR, RXRα and CAR are known to be involved in the induction of CYP3As. Here we report nuclear receptors PXR and RXRα but not CAR demonstrate gender- and age-dependent expression. Also, treatment of C57BL/6J mice with DEX induces PXR but not RXRα or CAR. In summary, we demonstrate DEX is not only able to up-regulate CYP3A expression and activity, but also the nuclear receptor PXR through which it may exert this effect. Furthermore, the gender- and age-dependent pattern of basal PXR and RXRα expression is similar to the 3 CYP3As analysed.

KW - CAR

KW - CYP3A11

KW - CYP3A13

KW - CYP3A25

KW - DEX

KW - Expression

KW - Induction

KW - Mouse

KW - PXR

KW - RXRα

UR - http://www.scopus.com/inward/record.url?scp=33845794361&partnerID=8YFLogxK

UR - https://www.sciencedirect.com/journal/archives-of-biochemistry-and-biophysics/vol/457/issue/1

U2 - 10.1016/j.abb.2006.09.017

DO - 10.1016/j.abb.2006.09.017

M3 - Article

C2 - 17107656

AN - SCOPUS:33845794361

SN - 0003-9861

VL - 457

SP - 105

EP - 110

JO - Archives of Biochemistry and Biophysics

JF - Archives of Biochemistry and Biophysics

IS - 1

ER -

Regulation and induction of CYP3A11, CYP3A13 and CYP3A25 in C57BL/6J mouse liver

Abstract

Keywords

Access to Document

Fingerprint

Cite this