Salivary proteins mediate greatest protection against dental erosion

Mahdi Amen Mohamed Mutahar, G. Carpenter, D. Bartlett, R. Moazzez

Research output: Contribution to conferenceAbstractpeer-review


Objectives: The aim is to investigate the effect of salivary ions and proteins on eroded enamel in a laboratory investigation.

Methods: 40 polished enamel specimens were prepared from extracted human teeth (Research ethics approval, Northampton REC, 14/EM/0183) and randomly assigned to 4 subgroups. 10 enamel samples per group were allocated to parotid, whole mouth, artificial saliva and water and immersed in the corresponding solution for 24 hours followed by a further 30 minutes prior to exposure to a 10-min erosion cycle in 80 ml of 0.3% pH 3.2, citric acid, agitated at room temperature, followed by 2-min water rinse. The 30 min immersion in the corresponding solution followed by the acid was repeated 5 times for all samples. Mean step height change from 5 randomly assigned points was measured using a non-contacting profilometer and Knoop microhardness measured at baseline (KHNb) and on the eroded surface of each sample (KHNe) and SMH change = (KHNb – KHNe) was calculated. Linear Regression model and Stata12.0 were used for the statistical analysis.

Results: Whole and parotid saliva produced significantly less step height (4.16±0.57 µm, 6.41±0.71 µm respectively) than artificial saliva (7.47±0.98µm) and these differences were statistically significant compared to water (10.89±0.98µm and p< 0.0001). Microhardness change, for whole mouth (224.11 ±29.29 KHN p<0.0001), parotid (208.16 ±50.20 KHN p<0.0001) and artificial saliva (194.0±19.75KHN p<0.002) was significantly greater than water (155.34±18.4 KHN). Whole mouth saliva had significantly greater microhardness change than artificial (p<0.012).

Conclusions: Saliva, containing proteins, appears to offer greater protection against dental erosion than artificial salvia and water. Whole mouth saliva provided less step height and greater hardness change than parotid saliva.
Original languageEnglish
Number of pages1
Publication statusPublished - 14 Sept 2015
EventThe British Society for Oral and Dental Research Annual Meeting – Cardiff, 14-16 September 2015: 2015 BSODR Annual General Meeting - Cardiff, United Kingdom
Duration: 14 Sept 201516 Sept 2015


ConferenceThe British Society for Oral and Dental Research Annual Meeting – Cardiff, 14-16 September 2015
Country/TerritoryUnited Kingdom
Internet address


Dive into the research topics of 'Salivary proteins mediate greatest protection against dental erosion'. Together they form a unique fingerprint.

Cite this