Screening of sulfate-reducing bacteria in colonoscopy samples from healthy and colitic human gut mucosa

Vitaly Zinkevich; I. Beech

doi:10.1111/j.1574-6941.2000.tb00764.x

Screening of sulfate-reducing bacteria in colonoscopy samples from healthy and colitic human gut mucosa

Vitaly Zinkevich, I. Beech

Research output: Contribution to journal › Article › peer-review

Abstract

A PCR-based approach combined with microbiological cultivation methods was employed to determine the occurrence of sulfate-reducing bacteria (SRB) in colon biopsy samples from ulcerative colitis patients and from non-colitic controls. The detection of mucosa-associated SRB was carried out by digoxigenin-dUTP-labelled PCR amplification, in liquid Postgate medium B and in a new liquid medium, termed VM medium I. Using Postgate medium B, the growth of SRB was confirmed in 92% of the colitic specimens and in 52% of non-colitic samples. However, PCR analysis and incubation in VM medium I detected SRB in 100% of biopsy material indicating ubiquitous presence of SRB in human colon mucosa.

Original language	English
Pages (from-to)	147-155
Number of pages	9
Journal	FEMS Microbiology Ecology
Volume	34
Issue number	2
DOIs	https://doi.org/10.1111/j.1574-6941.2000.tb00764.x
Publication status	Published - Dec 2000

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title = "Screening of sulfate-reducing bacteria in colonoscopy samples from healthy and colitic human gut mucosa",

abstract = "A PCR-based approach combined with microbiological cultivation methods was employed to determine the occurrence of sulfate-reducing bacteria (SRB) in colon biopsy samples from ulcerative colitis patients and from non-colitic controls. The detection of mucosa-associated SRB was carried out by digoxigenin-dUTP-labelled PCR amplification, in liquid Postgate medium B and in a new liquid medium, termed VM medium I. Using Postgate medium B, the growth of SRB was confirmed in 92\% of the colitic specimens and in 52\% of non-colitic samples. However, PCR analysis and incubation in VM medium I detected SRB in 100\% of biopsy material indicating ubiquitous presence of SRB in human colon mucosa.",

author = "Vitaly Zinkevich and I. Beech",

year = "2000",

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doi = "10.1111/j.1574-6941.2000.tb00764.x",

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AU - Zinkevich, Vitaly

AU - Beech, I.

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AB - A PCR-based approach combined with microbiological cultivation methods was employed to determine the occurrence of sulfate-reducing bacteria (SRB) in colon biopsy samples from ulcerative colitis patients and from non-colitic controls. The detection of mucosa-associated SRB was carried out by digoxigenin-dUTP-labelled PCR amplification, in liquid Postgate medium B and in a new liquid medium, termed VM medium I. Using Postgate medium B, the growth of SRB was confirmed in 92% of the colitic specimens and in 52% of non-colitic samples. However, PCR analysis and incubation in VM medium I detected SRB in 100% of biopsy material indicating ubiquitous presence of SRB in human colon mucosa.

U2 - 10.1111/j.1574-6941.2000.tb00764.x

DO - 10.1111/j.1574-6941.2000.tb00764.x

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JO - FEMS Microbiology Ecology

JF - FEMS Microbiology Ecology

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ER -

Screening of sulfate-reducing bacteria in colonoscopy samples from healthy and colitic human gut mucosa

Abstract

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