Solid-phase extraction in clinical biochemistry

V. Walker, Graham Mills

Research output: Contribution to journalArticlepeer-review

Abstract

In order to measure low concentrations of analytes in plasma and urine, it is often necessary to extract and concentrate them. With solid-phase extraction (SPE), this is achieved by partitioning the analytes between a solid and a liquid or headspace vapour. A wide range of high-quality materials is now available to do this, offering a variety of separation modes for different applications. These include partitioning using reversed-phase, normal-phase, ion-exchange, restricted-access and immunoaffinity sorbents or molecularly imprinted polymers and, increasingly, combinations of these processes. Solid-phase microextraction was introduced to analyse volatile and semi-volatile compounds. The range of sampling formats has expanded from simple packed syringes to cartridges, disks, SPE pipette tips and 96-well plates. These developments have facilitated automated off- and on-line sample processing. The basic principles of SPE and the recent innovations are reviewed here. This is a technological growth area. Some of the developments are finding application in clinical toxicology. However, they could also be of wider value in clinical chemistry - for example, for analyses of volatile and non-volatile metabolites, peptides, radioactive elements and trace metal speciation.
Original languageEnglish
Pages (from-to)464-477
Number of pages14
JournalAnnals of Clinical Biochemistry
Volume39
Issue number5
DOIs
Publication statusPublished - Sep 2002

Fingerprint

Dive into the research topics of 'Solid-phase extraction in clinical biochemistry'. Together they form a unique fingerprint.

Cite this