Spatially offset Raman spectroscopy - how deep?

Sara Mosca, Priyanka Dey, Marzieh Salimi, Benjamin Gardner, Francesca Palombo, Nick Stone, Pavel Matousek*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

26 Downloads (Pure)


Spatially offset Raman spectroscopy (SORS) is a technique for interrogating the subsurface composition of turbid samples noninvasively. This study generically addresses a fundamental question relevant to a wide range of SORS studies, which is how deep SORS probes for any specific spatial offset when analyzing a turbid sample or, in turn, what magnitude of spatial offset one should select to probe a specific depth. This issue is addressed by using Monte Carlo simulations, under the assumption of negligible absorption, which establishes that the key parameter governing the extent of the probed zone for a point-like illumination and point-like collection SORS geometry is the reduced scattering coefficient of the medium. This can either be deduced from literature data or directly estimated from a SORS measurement by evaluating the Raman intensity profile from multiple spatial offsets. Once this is known, the extent of the probed zone can be determined for any specific SORS spatial offset using the Monte Carlo simulation results presented here. The proposed method was tested using experimental data on stratified samples by analyzing the signal detected from a thin layer that was moved through a stack of layers using both non-absorbing and absorbing samples. The proposed simple methodology provides important additional information on SORS measurements with direct relevance to a wide range of SORS applications including biomedical, pharmaceutical, security, forensics, and cultural heritage.

Original languageEnglish
Pages (from-to)6755-6762
Number of pages8
JournalAnalytical Chemistry
Issue number17
Early online date22 Apr 2021
Publication statusPublished - 4 May 2021


  • UKRI
  • EP/R020965/1


Dive into the research topics of 'Spatially offset Raman spectroscopy - how deep?'. Together they form a unique fingerprint.

Cite this