Statistical optimization for lipase production from solid waste of vegetable oil industry

Rajesh Kumar Sahoo; Mohit Kumar; Swati Mohanty; Matthew Sawyer; Pattanathu K. S. M. Rahman; Lala Behari Sukla; Enketeswara Subudhi

doi:10.1080/10826068.2018.1431785

Statistical optimization for lipase production from solid waste of vegetable oil industry

Rajesh Kumar Sahoo, Mohit Kumar, Swati Mohanty, Matthew Sawyer, Pattanathu K. S. M. Rahman, Lala Behari Sukla, Enketeswara Subudhi

Institute of Life Sciences and Healthcare

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Abstract

The production of biofuel using thermostable bacterial lipase from hot spring bacteria out of low-cost agricultural residue olive oil cake is reported in the present paper. Using a lipase enzyme from Bacillus licheniformis, a 66.5% yield of methyl esters was obtained. Optimum parameters were determined, with maximum production of lipase at a pH of 8.2, temperature 50.8°C, moisture content of 55.7%, and biosurfactant content of 1.693 mg. The contour plots and 3D surface responses depict the significant interaction of pH and moisture content with biosurfactant during lipase production. Chromatographic analysis of the lipase transesterification product was methyl esters, from kitchen waste oil under optimized conditions, generated methyl palmitate, methyl stearate, methyl oleate, and methyl linoleate.

Original language	English
Pages (from-to)	321-326
Journal	Preparative Biochemistry and Biotechnology
Volume	48
Issue number	4
Early online date	30 Mar 2018
DOIs	https://doi.org/10.1080/10826068.2018.1431785
Publication status	Published - Apr 2018

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10.1080/10826068.2018.1431785

Statistical optimization for lipase production
This is an Accepted Manuscript of an article published by Taylor & Francis in Preparative Biochemistry and Biotechnology on 30/03/2018, available online: http://www.tandfonline.com/10.1080/10826068.2018.1431785.
Accepted author manuscript (Post-print), 758 KB

https://www.tandfonline.com/doi/full/10.1080/10826068.2018.1431785

Supplemental material for 'Statistical optimization for lipase production from solid waste of vegetable oil industry'.
Sahoo, R. K. (Creator), Kumar, M. (Creator), Mohanty, S. (Creator), Sawyer, M. (Creator), Rahman, P. (Creator), Sukla, L. B. (Creator) & Subudhi, E. (Creator), Taylor & Francis, 30 Mar 2018
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Siksha ‘O’ Anusandhan University
Pattanathu Rahman (Visiting researcher)
2014
Activity: Visiting an external organisation types › Visiting an external academic institution

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title = "Statistical optimization for lipase production from solid waste of vegetable oil industry",

abstract = "The production of biofuel using thermostable bacterial lipase from hot spring bacteria out of low-cost agricultural residue olive oil cake is reported in the present paper. Using a lipase enzyme from Bacillus licheniformis, a 66.5% yield of methyl esters was obtained. Optimum parameters were determined, with maximum production of lipase at a pH of 8.2, temperature 50.8°C, moisture content of 55.7%, and biosurfactant content of 1.693 mg. The contour plots and 3D surface responses depict the significant interaction of pH and moisture content with biosurfactant during lipase production. Chromatographic analysis of the lipase transesterification product was methyl esters, from kitchen waste oil under optimized conditions, generated methyl palmitate, methyl stearate, methyl oleate, and methyl linoleate.",

author = "Sahoo, {Rajesh Kumar} and Mohit Kumar and Swati Mohanty and Matthew Sawyer and Rahman, {Pattanathu K. S. M.} and Sukla, {Lala Behari} and Enketeswara Subudhi",

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doi = "10.1080/10826068.2018.1431785",

language = "English",

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T1 - Statistical optimization for lipase production from solid waste of vegetable oil industry

AU - Sahoo, Rajesh Kumar

AU - Kumar, Mohit

AU - Mohanty, Swati

AU - Sawyer, Matthew

AU - Rahman, Pattanathu K. S. M.

AU - Sukla, Lala Behari

AU - Subudhi, Enketeswara

PY - 2018/4

Y1 - 2018/4

N2 - The production of biofuel using thermostable bacterial lipase from hot spring bacteria out of low-cost agricultural residue olive oil cake is reported in the present paper. Using a lipase enzyme from Bacillus licheniformis, a 66.5% yield of methyl esters was obtained. Optimum parameters were determined, with maximum production of lipase at a pH of 8.2, temperature 50.8°C, moisture content of 55.7%, and biosurfactant content of 1.693 mg. The contour plots and 3D surface responses depict the significant interaction of pH and moisture content with biosurfactant during lipase production. Chromatographic analysis of the lipase transesterification product was methyl esters, from kitchen waste oil under optimized conditions, generated methyl palmitate, methyl stearate, methyl oleate, and methyl linoleate.

AB - The production of biofuel using thermostable bacterial lipase from hot spring bacteria out of low-cost agricultural residue olive oil cake is reported in the present paper. Using a lipase enzyme from Bacillus licheniformis, a 66.5% yield of methyl esters was obtained. Optimum parameters were determined, with maximum production of lipase at a pH of 8.2, temperature 50.8°C, moisture content of 55.7%, and biosurfactant content of 1.693 mg. The contour plots and 3D surface responses depict the significant interaction of pH and moisture content with biosurfactant during lipase production. Chromatographic analysis of the lipase transesterification product was methyl esters, from kitchen waste oil under optimized conditions, generated methyl palmitate, methyl stearate, methyl oleate, and methyl linoleate.

UR - https://research.tees.ac.uk/en/publications/statistical-optimization-for-lipase-production-from-solid-waste-o

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DO - 10.1080/10826068.2018.1431785

M3 - Article

SN - 1082-6068

VL - 48

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EP - 326

JO - Preparative Biochemistry and Biotechnology

JF - Preparative Biochemistry and Biotechnology

IS - 4

ER -

Statistical optimization for lipase production from solid waste of vegetable oil industry

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Supplemental material for 'Statistical optimization for lipase production from solid waste of vegetable oil industry'.

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Siksha ‘O’ Anusandhan University

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