Sulfhydryl free radical formation enzymatically by sonolysis, by radiolysis, and thermally: Vitamin A, curcumin, muconic acid, and related conjugated olefins as references

Massimo D'Aquino, Christopher Bullion, Mridula Chopra, Damayanti Devi, Sunitibala Devi, Christina Dunster, Gary James, Erika Komuro, Subhas Kundu, Etsuo Niki, Farah Raza, Forbes Robertson, Jiten Sharma, Robin Willson

Research output: Chapter in Book/Report/Conference proceedingChapter (peer-reviewed)peer-review

Abstract

Sulfhydryl (thiyl) free radicals can be generated in a variety of biochemical systems, particularly those containing a peroxidase or myoglobin and hydrogen peroxide. They have long been known to be formed thermally, by photolysis, and by radiolysis. They can also be readily formed by sonolysis in systems, such as thiyl free radicals can rapidly undergo electron transfer and hydrogen transfer reactions with a variety of biological molecules. They can also undergo addition reactions with oxygen and with several conjugated olefinic and aromatic compounds including vitamins A and D, styrene, and several polyaromatic dihydrodiols. This chapter discusses the use of vitamin A, the food additive curcumin, and the benzene metabolite muconic acid as reference models in studies of thiyl free radical reactivity together with some of the methods by which thiyl free radicals are generated in the laboratory. Sonolysis and other studies, with curcumin or β-carotene in particular, provide stimulating, relatively inexpensive, and practical teaching excercises.

Original languageEnglish
Title of host publicationOxygen Radicals in Biological Systems Part C
EditorsLester Packer
PublisherElsevier
Pages34-46
Number of pages13
ISBN (Print)9780121821340
DOIs
Publication statusPublished - 1 Jan 1994

Publication series

NameMethods in Enzymology
Volume233
ISSN (Print)0076-6879

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