TY - CHAP
T1 - Sulfhydryl free radical formation enzymatically by sonolysis, by radiolysis, and thermally
T2 - Vitamin A, curcumin, muconic acid, and related conjugated olefins as references
AU - D'Aquino, Massimo
AU - Bullion, Christopher
AU - Chopra, Mridula
AU - Devi, Damayanti
AU - Devi, Sunitibala
AU - Dunster, Christina
AU - James, Gary
AU - Komuro, Erika
AU - Kundu, Subhas
AU - Niki, Etsuo
AU - Raza, Farah
AU - Robertson, Forbes
AU - Sharma, Jiten
AU - Willson, Robin
N1 - Funding Information:
Financial support from the Association for International Cancer Research and helpful discussions with Dr. Francis Duck regarding sonolysis and with Professor Enrique Cadenas and Cecelia Giulivi regarding the myoglobin system are gratefully acknowledged.
PY - 1994/1/1
Y1 - 1994/1/1
N2 - Sulfhydryl (thiyl) free radicals can be generated in a variety of biochemical systems, particularly those containing a peroxidase or myoglobin and hydrogen peroxide. They have long been known to be formed thermally, by photolysis, and by radiolysis. They can also be readily formed by sonolysis in systems, such as thiyl free radicals can rapidly undergo electron transfer and hydrogen transfer reactions with a variety of biological molecules. They can also undergo addition reactions with oxygen and with several conjugated olefinic and aromatic compounds including vitamins A and D, styrene, and several polyaromatic dihydrodiols. This chapter discusses the use of vitamin A, the food additive curcumin, and the benzene metabolite muconic acid as reference models in studies of thiyl free radical reactivity together with some of the methods by which thiyl free radicals are generated in the laboratory. Sonolysis and other studies, with curcumin or β-carotene in particular, provide stimulating, relatively inexpensive, and practical teaching excercises.
AB - Sulfhydryl (thiyl) free radicals can be generated in a variety of biochemical systems, particularly those containing a peroxidase or myoglobin and hydrogen peroxide. They have long been known to be formed thermally, by photolysis, and by radiolysis. They can also be readily formed by sonolysis in systems, such as thiyl free radicals can rapidly undergo electron transfer and hydrogen transfer reactions with a variety of biological molecules. They can also undergo addition reactions with oxygen and with several conjugated olefinic and aromatic compounds including vitamins A and D, styrene, and several polyaromatic dihydrodiols. This chapter discusses the use of vitamin A, the food additive curcumin, and the benzene metabolite muconic acid as reference models in studies of thiyl free radical reactivity together with some of the methods by which thiyl free radicals are generated in the laboratory. Sonolysis and other studies, with curcumin or β-carotene in particular, provide stimulating, relatively inexpensive, and practical teaching excercises.
UR - http://www.scopus.com/inward/record.url?scp=0028237214&partnerID=8YFLogxK
U2 - 10.1016/S0076-6879(94)33006-9
DO - 10.1016/S0076-6879(94)33006-9
M3 - Chapter (peer-reviewed)
AN - SCOPUS:0028237214
SN - 9780121821340
T3 - Methods in Enzymology
SP - 34
EP - 46
BT - Oxygen Radicals in Biological Systems Part C
A2 - Packer, Lester
PB - Elsevier
ER -