TA cloning approaches to cloning DNA with damaged ends DNA

    Research output: Chapter in Book/Report/Conference proceedingChapter (peer-reviewed)peer-review

    Abstract

    DNA ends can become damaged for various reasons making them unsuitable for TA cloning techniques, the easiest and most common of the DNA cloning technologies. Examples of end-damaged DNA include ancient DNA and those produced by laboratory methods such as sonication. In this chapter, we discuss how to deal with end-damaged DNA prior to cloning with either the popular pGEM®-T Easy Vector Systems Kit and TOPO™ TA Cloning™ Kits.
    Original languageEnglish
    Title of host publicationDNA Manipulation and Analysis
    EditorsGarry Scarlett
    Place of PublicationNew York
    PublisherHumana Press
    Chapter5
    Pages55-64
    Number of pages9
    Edition1st
    ISBN (Electronic)9781071630044
    ISBN (Print)9781071630037
    DOIs
    Publication statusPublished - 1 Mar 2023

    Publication series

    NameMethods in Molecular Biology
    Volume2633
    ISSN (Print)1064-3745
    ISSN (Electronic)1940-6029

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