Tendon reattachment to bone in an ovine tendon defect model of retraction using allogenic and xenogenic demineralised bone matrix incorporated with mesenchymal stem cells

Tanujan Thangarajah, Shirin Shahbazi, Catherine J. Pendegrass, Simon Lambert, Susan Alexander, Gordon W. Blunn

Research output: Contribution to journalArticlepeer-review

80 Downloads (Pure)

Abstract

Background: Tendon-bone healing following rotator cuff repairs is mainly impaired by poor tissue quality. Demineralised bone matrix promotes healing of the tendon-bone interface but its role in the treatment of tendon tears with retraction has not been investigated. We hypothesized that cortical demineralised bone matrix used with minimally manipulated mesenchymal stem cells will result in improved function and restoration of the tendon-bone interface with no difference between xenogenic and allogenic scaffolds.

Materials and methods: In an ovine model, the patellar tendon was detached from the tibial tuberosity and a complete distal tendon transverse defect measuring 1 cm was created. Suture anchors were used to reattach the tendon and xenogenic demineralised bone matrix + minimally manipulated mesenchymal stem cells (n = 5), or allogenic demineralised bone matrix + minimally manipulated mesenchymal stem cells (n = 5) were used to bridge the defect. Graft incorporation into the tendon and its effect on regeneration of the enthesis was assessed using histomorphometry. Force plate analysis was used to assess functional recovery.

Results: Compared to the xenograft, the allograft was associated with significantly higher functional weight bearing at 6 (P = 0.047), 9 (P = 0.028), and 12 weeks (P = 0.009). In the allogenic group this was accompanied by greater remodeling of the demineralised bone matrix into tendon-like tissue in the region of the defect (p = 0.015), and a more direct type of enthesis characterized by significantly more fibrocartilage (p = 0.039). No failures of tendon-bone healing were noted in either group.

Conslusion: Demineralised bone matrix used with minimally manipulated mesenchymal stem cells promotes healing of the tendon-bone interface in an ovine model of acute tendon retraction, with superior mechanical and histological results associated with use of an allograft.

Original languageEnglish
Article numbere0161473
Number of pages14
JournalPLoS One
Volume11
Issue number9
DOIs
Publication statusPublished - 8 Sep 2016

Keywords

  • animals
  • biomechanical phenomena
  • bone matrix
  • bone and bones
  • disease models, animal
  • female
  • heterografts
  • mesenchymal stromal cells
  • patellar ligament
  • sheep
  • tendons
  • tomography, X-Ray computed
  • transplantation, homologous
  • weight-bearing
  • RCUK
  • EPSRC Proof of Concept Grant

Fingerprint

Dive into the research topics of 'Tendon reattachment to bone in an ovine tendon defect model of retraction using allogenic and xenogenic demineralised bone matrix incorporated with mesenchymal stem cells'. Together they form a unique fingerprint.

Cite this