The construction of customised nucleosomal arrays

Chenyi Wu, Christopher Read, John Mcgeehan, Colyn Crane-robinson

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Abstract

A simple, efficient and reliable method is demonstrated for cloning long tandem arrays of the 601 nucleosomal positioning sequence. Additionally, it is shown that such long arrays can be ligated together in vitro with high efficiency. By combining these two procedures it becomes straightforward to synthesise customised arrays that contain different (or variable) nucleosomal repeat lengths (NRLs) and monosome units bearing chemical modifications such as fluorophores, methyl groups or reaction sites. This is therefore an enabling technology for the in vitro study of chromatin structure and function.
Original languageEnglish
Pages (from-to)71-75
Number of pages5
JournalAnalytical Biochemistry
Volume496
Early online date17 Dec 2015
DOIs
Publication statusPublished - 1 Mar 2016

Keywords

  • chromatin
  • nucleosome
  • 30 nm fibre
  • 601 sequence
  • RCUK
  • E500595/1

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