A simple, efficient and reliable method is demonstrated for cloning long tandem arrays of the 601 nucleosomal positioning sequence. Additionally, it is shown that such long arrays can be ligated together in vitro with high efficiency. By combining these two procedures it becomes straightforward to synthesise customised arrays that contain different (or variable) nucleosomal repeat lengths (NRLs) and monosome units bearing chemical modifications such as fluorophores, methyl groups or reaction sites. This is therefore an enabling technology for the in vitro study of chromatin structure and function.
- 30 nm fibre
- 601 sequence