The construction of customised nucleosomal arrays

Chenyi Wu; Christopher Read; John Mcgeehan; Colyn Crane-robinson

doi:10.1016/j.ab.2015.11.018

The construction of customised nucleosomal arrays

Chenyi Wu, Christopher Read, John Mcgeehan, Colyn Crane-robinson

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Abstract

A simple, efficient and reliable method is demonstrated for cloning long tandem arrays of the 601 nucleosomal positioning sequence. Additionally, it is shown that such long arrays can be ligated together in vitro with high efficiency. By combining these two procedures it becomes straightforward to synthesise customised arrays that contain different (or variable) nucleosomal repeat lengths (NRLs) and monosome units bearing chemical modifications such as fluorophores, methyl groups or reaction sites. This is therefore an enabling technology for the in vitro study of chromatin structure and function.

Original language	English
Pages (from-to)	71-75
Number of pages	5
Journal	Analytical Biochemistry
Volume	496
Early online date	17 Dec 2015
DOIs	https://doi.org/10.1016/j.ab.2015.11.018
Publication status	Published - 1 Mar 2016

Keywords

chromatin
nucleosome
30 nm fibre
601 sequence
RCUK
E500595/1

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The_construction_of_customisedAccepted author manuscript (Post-print), 1.1 MBLicence: CC BY-NC-ND

http://linkinghub.elsevier.com/retrieve/pii/S0003269715005448

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abstract = "A simple, efficient and reliable method is demonstrated for cloning long tandem arrays of the 601 nucleosomal positioning sequence. Additionally, it is shown that such long arrays can be ligated together in vitro with high efficiency. By combining these two procedures it becomes straightforward to synthesise customised arrays that contain different (or variable) nucleosomal repeat lengths (NRLs) and monosome units bearing chemical modifications such as fluorophores, methyl groups or reaction sites. This is therefore an enabling technology for the in vitro study of chromatin structure and function.",

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N2 - A simple, efficient and reliable method is demonstrated for cloning long tandem arrays of the 601 nucleosomal positioning sequence. Additionally, it is shown that such long arrays can be ligated together in vitro with high efficiency. By combining these two procedures it becomes straightforward to synthesise customised arrays that contain different (or variable) nucleosomal repeat lengths (NRLs) and monosome units bearing chemical modifications such as fluorophores, methyl groups or reaction sites. This is therefore an enabling technology for the in vitro study of chromatin structure and function.

AB - A simple, efficient and reliable method is demonstrated for cloning long tandem arrays of the 601 nucleosomal positioning sequence. Additionally, it is shown that such long arrays can be ligated together in vitro with high efficiency. By combining these two procedures it becomes straightforward to synthesise customised arrays that contain different (or variable) nucleosomal repeat lengths (NRLs) and monosome units bearing chemical modifications such as fluorophores, methyl groups or reaction sites. This is therefore an enabling technology for the in vitro study of chromatin structure and function.

KW - chromatin

KW - nucleosome

KW - 30 nm fibre

KW - 601 sequence

KW - RCUK

KW - E500595/1

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DO - 10.1016/j.ab.2015.11.018

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JO - Analytical Biochemistry

JF - Analytical Biochemistry

ER -

The construction of customised nucleosomal arrays

Abstract

Keywords

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