The effect of adherens junction components on keratinocyte adhesion in vitro: potential implications for sealing the skin-implant interface of intraosseous transcutaneous amputation prostheses

Catherine Jane Pendegrass, Bethan Tucker, Shelain Patel, Robert Dowling, Gordon William Blunn

Research output: Contribution to journalArticlepeer-review

Abstract

Amputation places a significant burden on healthcare systems worldwide as patients suffer life-long complications associated with the stump-socket interface. Skin penetrating, osseointegrated implants like intraosseous transcutaneous amputation prostheses, could overcome this, however, they rely on the formation and maintenance of an infection-free seal at the skin-implant interface. Epithelial cell migration around transcutaneous implants creates downgrowth, which leads to infection and implant failure. Epithelial cells form cell-cell attachments via adherens junctions and desmosomes that prevent cell migration via contact inhibition. If epithelial cells formed cell-cell attachments with an implant surface, it could facilitate stronger cell attachment and prevent downgrowth. In adherens junctions, E-cadherin is essential in homotypic cell attachment. In this study, we have demonstrated that cell-cell adherens junctions can be formed on substrates adsorbed with E-cadherin. We have assessed the effects of two E-cadherin peptides and determined an optimal concentration for increasing cell attachment via adherens junctions. We have demonstrated that adsorption of 15 μg/mL of the full extracellular domain of E-cadherin to titanium alloy significantly increases metabolic activity, cell area, and attachment of murine keratinocytes in vitro, with a fourfold increase in attachment via adherens junctions at 24, 48, and 72 h.

Original languageEnglish
Pages (from-to)3463-71
Number of pages9
JournalJournal of Biomedical Materials Research Part A
Volume100
Issue number12
DOIs
Publication statusPublished - Dec 2012

Keywords

  • Adherens Junctions
  • Amputation Stumps
  • Animals
  • Cadherins
  • Cell Adhesion
  • Keratinocytes
  • Mice
  • Prostheses and Implants
  • Skin
  • Time Factors
  • Vinculin
  • beta Catenin
  • Research Support, N.I.H., Extramural

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