The effect of glutaraldehyde on the development of marine biofilms formed on surfaces of AISI 304 stainless steel

R. C. Tapper, J. R. Smith, I. B. Beech, M. R. Viera, P. S. Guiamet, H. Videla, C. L. Swords, R. G. J. Edyvean

Research output: Chapter in Book/Report/Conference proceedingConference contribution


The effect of pre-conditioning polished and unpolished AISI 304 stainless steel surfaces with glutaraldehyde on the attachment, growth and morphology of an aerobic consortium of marine bacteria was investigated using total cell number counts, epifluorescence microscopy, Atomic Force Microscopy (AFM), Environmental Scanning Electron Microscopy (ESEM) and grazing-angle Fourier Transform Infrared (FÜR) spectroscopy. Both fully hydrated and dehydrated biofilms were studied using AFM and ESEM. Formation of the conditioning layer on steel surfaces from the culture medium, in the presence and absence of glutaraldehyde was monitored in-situ employing AFM and Grazing Angle FT1R spectroscopy. The influence of both surface area and surface energy upon the numbers of bacteria attached to polished and unpolished coupons was determined. This study has shown the influence of pretreatment of AISI 304 stainless steel with glutaraldehyde upon biofilm formation and has demonstrated the ability of AFM, ESEM and FTIR to be used as valuable tools for the in-situ investigation of the effect of biocides on bacterial biofilms.

Original languageEnglish
Title of host publicationCorrosion '97, 9-14 March, New Orleans, Louisiana
PublisherNACE International
Publication statusPublished - Mar 1997
EventCorrosion 1997 - New Orleans, United States
Duration: 9 Mar 199714 Mar 1997

Publication series

NameInternational Corrosion Conference Series
ISSN (Print)0361-4409


ConferenceCorrosion 1997
Country/TerritoryUnited States
CityNew Orleans


  • Atomic force microscopy
  • Bacterial attachment
  • Bacterial consortia
  • Biocides
  • Environmental scanning electron microscopy
  • Glutaraldehyde
  • Marine biofilms
  • Stainless steel


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