TY - JOUR
T1 - The effects of acute ethanolamine administration on isoprenaline-induced myocardial infarction in adult Wistar rats
AU - Garson, Christie
AU - Gwanyanya, Asfree
AU - Bugarith, Kishor
AU - Kelly-Laubscher, Roisin
N1 - Abstracts from the 2012 Annual Meeting of the International Society for Heart Research North American Section
PY - 2012/8
Y1 - 2012/8
N2 - Cardiovascular stress, such as that due to the synthetic catecholamine,
isoprenaline (iso), can induce myocardial infarction (MI).
Ethanolamine (etn) is a biogenic amine, found in certain food
types, that protects the isolated rat heart against ischemiareperfusion
injury. The aim of this study was to investigate the
cardioprotective effects of acute etn on iso-induced MI. Male Wistar
rats (250-350 g) were divided into four groups: Control (saline
injections only), iso + etn (iso 67 mg/kg, s.c. and etn 10 mg/
kg i.p.), iso (67 mg/kg, s.c. and a saline injection), and etn (saline injection
and 10 mg/kg i.p.). Rats were anaesthetised with sodium
pentobarbitone (60 mg/kg, i.p.) and mechanically ventilated with
room air. Arterial blood pressure wasmeasured from the right carotid
artery and electrocardiogram (ECG) was recorded from surface
leads. Infarct size was measured using triphenyltetrazolium chloride
(TTC) staining and the by-products of lipid peroxidation were quantified.
Iso administration significantly increased the infarct size
(62.0±3.9%, Pb0.001) (mean±SEM) compared to control rats
(23.7±2.7%). The heart weight/body weight (HW/BW) ratio was
also significantly raised in iso rats (4.6±0.1, Pb0.001) compared
to control rats (3.4±0.02). Iso + etn (5.0±0.2 Pb0.05) treated
rats displayed significantly greater HW/BW ratio compared to
iso treated rats. However, there was no significant difference in
infarct size between iso and iso + etn. Iso + etn treated rats
had a lower death rate (1.9%) compared to iso rats (11.3%).
There was a significant increase in the amount of conjugated dienes
(CD) in iso rats (59.56±2.6 μmol/L) compared to control
rats (39.9±4.0 μmol/L, Pb0.05). There was no significant difference
in the generation of CDs between control and iso + etn
rats. In summary, etn seems to protect the rat from iso-induced
MI; however the possibility of increased cardiac hypertrophy
needs further investigation.
AB - Cardiovascular stress, such as that due to the synthetic catecholamine,
isoprenaline (iso), can induce myocardial infarction (MI).
Ethanolamine (etn) is a biogenic amine, found in certain food
types, that protects the isolated rat heart against ischemiareperfusion
injury. The aim of this study was to investigate the
cardioprotective effects of acute etn on iso-induced MI. Male Wistar
rats (250-350 g) were divided into four groups: Control (saline
injections only), iso + etn (iso 67 mg/kg, s.c. and etn 10 mg/
kg i.p.), iso (67 mg/kg, s.c. and a saline injection), and etn (saline injection
and 10 mg/kg i.p.). Rats were anaesthetised with sodium
pentobarbitone (60 mg/kg, i.p.) and mechanically ventilated with
room air. Arterial blood pressure wasmeasured from the right carotid
artery and electrocardiogram (ECG) was recorded from surface
leads. Infarct size was measured using triphenyltetrazolium chloride
(TTC) staining and the by-products of lipid peroxidation were quantified.
Iso administration significantly increased the infarct size
(62.0±3.9%, Pb0.001) (mean±SEM) compared to control rats
(23.7±2.7%). The heart weight/body weight (HW/BW) ratio was
also significantly raised in iso rats (4.6±0.1, Pb0.001) compared
to control rats (3.4±0.02). Iso + etn (5.0±0.2 Pb0.05) treated
rats displayed significantly greater HW/BW ratio compared to
iso treated rats. However, there was no significant difference in
infarct size between iso and iso + etn. Iso + etn treated rats
had a lower death rate (1.9%) compared to iso rats (11.3%).
There was a significant increase in the amount of conjugated dienes
(CD) in iso rats (59.56±2.6 μmol/L) compared to control
rats (39.9±4.0 μmol/L, Pb0.05). There was no significant difference
in the generation of CDs between control and iso + etn
rats. In summary, etn seems to protect the rat from iso-induced
MI; however the possibility of increased cardiac hypertrophy
needs further investigation.
U2 - 10.1016/j.yjmcc.2012.06.013
DO - 10.1016/j.yjmcc.2012.06.013
M3 - Meeting Abstract
SN - 0022-2828
VL - 53
SP - S3
JO - Journal of Molecular and Cellular Cardiology
JF - Journal of Molecular and Cellular Cardiology
IS - 2 Supp
T2 - Annual Meeting of the International Society for Heart Research North American Section
Y2 - 28 May 2012 through 31 May 2012
ER -
