@article{7ae9ed8c96e543689822b99e14d5a6a3,
title = "The formation and displacement of ordered DNA triplexes in self-assembled three-dimensional DNA crystals",
abstract = "Reconfigurable structures engineered through DNA hybridization and self-assembly offer both structural and dynamic applications in nanotechnology. Here, we have demonstrated that strand displacement of triplex-forming oligonucleotides (TFOs) can be translated to a robust macroscopic DNA crystal by coloring the crystals with covalently attached fluorescent dyes. We show that three different types of triplex strand displacement are feasible within the DNA crystals and the bound TFOs can be removed and/or replaced by (a) changing the pH from 5 to 7, (b) the addition of the Watson-Crick complement to a TFO containing a short toehold, and (c) the addition of a longer TFO that uses the duplex edge as a toehold. We have also proved by X-ray diffraction that the structure of the crystals remains as designed in the presence of the TFOs.",
author = "Yue Zhao and Chandrasekaran, {Arun Richard} and Rusling, {David A.} and Karol Woloszyn and Yudong Hao and Carina Hernandez and Simon Vecchioni and Ohayon, {Yoel P.} and Chengde Mao and Seeman, {Nadrian C.} and Ruojie Sha",
note = "Funding Information: We acknowledge support of the following grants to N.C.S. and R.S.: N000141912596 from the Office of Naval Research, DE-SC0007991 from the Department of Energy, and CCF-2106790 from NSF. This work was also supported by NSF (CCF-2107393 to C.M.). We thank Professor James W. Canary of NYU for discussion and support. We thank J. Yoder, A. Mulichak, E. Zoellner, and J. Digilio at IMCA-CAT of the Advanced Photon Source, Argonne National Laboratory, for their assistance with diffraction experiments. Publisher Copyright: {\textcopyright} 2023 American Chemical Society.",
year = "2023",
month = feb,
day = "2",
doi = "10.1021/jacs.2c12667",
language = "English",
volume = "145",
pages = "3599–3605",
journal = "Journal of the American Chemical Society",
issn = "0002-7863",
publisher = "American Chemical Society",
number = "6",
}