TY - JOUR
T1 - The UBAP1 Subunit of ESCRT-I Interacts with Ubiquitin via a SOUBA Domain
AU - Agromayor, Monica
AU - Soler, Nicolas
AU - Caballe, Anna
AU - Kueck, Tonya
AU - Freund, Stefan M.
AU - Allen, Mark D.
AU - Bycroft, Mark
AU - Perisic, Olga
AU - Ye, Yu
AU - Mcdonald, Bethan
AU - Scheel, Hartmut
AU - Hofmann, Kay
AU - Neil, Stuart J.D.
AU - Martin-Serrano, Juan
AU - Williams, Roger l.
PY - 2012/3/7
Y1 - 2012/3/7
N2 - The endosomal sorting complexes required for transport (ESCRTs) facilitate endosomal sorting of ubiquitinated cargo, MVB biogenesis, late stages of cytokinesis, and retroviral budding. Here we show that ubiquitin associated protein 1 (UBAP1), a subunit of human ESCRT-I, coassembles in a stable 1:1:1:1 complex with Vps23/TSG101, VPS28, and VPS37. The X-ray crystal structure of the C-terminal region of UBAP1 reveals a domain that we describe as a solenoid of overlapping UBAs (SOUBA). NMR analysis shows that each of the three rigidly arranged overlapping UBAs making up the SOUBA interact with ubiquitin. We demonstrate that UBAP1-containing ESCRT-I is essential for degradation of antiviral cell-surface proteins, such as tetherin (BST-2/CD317), by viral countermeasures, namely, the HIV-1 accessory protein Vpu and the Kaposi sarcoma-associated herpesvirus (KSHV) ubiquitin ligase K5.
AB - The endosomal sorting complexes required for transport (ESCRTs) facilitate endosomal sorting of ubiquitinated cargo, MVB biogenesis, late stages of cytokinesis, and retroviral budding. Here we show that ubiquitin associated protein 1 (UBAP1), a subunit of human ESCRT-I, coassembles in a stable 1:1:1:1 complex with Vps23/TSG101, VPS28, and VPS37. The X-ray crystal structure of the C-terminal region of UBAP1 reveals a domain that we describe as a solenoid of overlapping UBAs (SOUBA). NMR analysis shows that each of the three rigidly arranged overlapping UBAs making up the SOUBA interact with ubiquitin. We demonstrate that UBAP1-containing ESCRT-I is essential for degradation of antiviral cell-surface proteins, such as tetherin (BST-2/CD317), by viral countermeasures, namely, the HIV-1 accessory protein Vpu and the Kaposi sarcoma-associated herpesvirus (KSHV) ubiquitin ligase K5.
U2 - 10.1016/j.str.2011.12.013
DO - 10.1016/j.str.2011.12.013
M3 - Article
SN - 0969-2126
VL - 20
SP - 414
EP - 428
JO - Structure
JF - Structure
IS - 3
ER -
