The X-ray structure of L-threonine dehydrogenase from the common hospital pathogen              Clostridium difficile

Eyram Adjogatse; Josh Bennett; Jingxu Guo; Peter T. Erskine; Steve P. Wood; Brendan W. Wren; Jonathan B. Cooper

doi:10.1107/S2053230X21007135

The X-ray structure of _L-threonine dehydrogenase from the common hospital pathogen Clostridium difficile

Eyram Adjogatse, Josh Bennett, Jingxu Guo, Peter T. Erskine, Steve P. Wood, Brendan W. Wren, Jonathan B. Cooper^*

^*Corresponding author for this work

School of Biological Sciences

Research output: Contribution to journal › Article › peer-review

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Abstract

In many prokaryotes, the first step of threonine metabolism is catalysed by the enzyme threonine dehydrogenase (TDH), which uses NAD+ to oxidize its substrate to 2-amino-3-ketobutyrate. The absence of a functional TDH gene in humans suggests that inhibitors of this enzyme may have therapeutic potential against pathogens which are reliant on this enzyme. Here, TDH from Clostridium difficile has been cloned and overexpressed, and the X-ray structure of the apoenzyme form has been determined at 2.6 Å resolution.

Original language	English
Pages (from-to)	269-274
Journal	Acta Crystallographica Section F Structural Biology Communications
Volume	77
Issue number	8
DOIs	https://doi.org/10.1107/S2053230X21007135
Publication status	Published - 1 Aug 2021

Keywords

threonine dehydrogenase
Clostridium difficile
protein crystallography
molecular replacement
refinement

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10.1107/S2053230X21007135

X-ray structure
Adjogatse, E., Bennett, J., Guo, J., Erskine, P. T., Wood, S. P., Wren, B. W., & Cooper, J. B. (2021). The X-ray structure of l-threonine dehydrogenase from the common hospital pathogen Clostridium difficile. Acta Crystallographica Section F: Structural Biology Communications, 77(8). https://doi.org/10.1107/S2053230X21007135
Final published version, 1.54 MB

Cite this

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title = "The X-ray structure of L-threonine dehydrogenase from the common hospital pathogen Clostridium difficile",

abstract = "In many prokaryotes, the first step of threonine metabolism is catalysed by the enzyme threonine dehydrogenase (TDH), which uses NAD+ to oxidize its substrate to 2-amino-3-ketobutyrate. The absence of a functional TDH gene in humans suggests that inhibitors of this enzyme may have therapeutic potential against pathogens which are reliant on this enzyme. Here, TDH from Clostridium difficile has been cloned and overexpressed, and the X-ray structure of the apoenzyme form has been determined at 2.6 {\AA} resolution.",

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AU - Adjogatse, Eyram

AU - Bennett, Josh

AU - Guo, Jingxu

AU - Erskine, Peter T.

AU - Wood, Steve P.

AU - Wren, Brendan W.

AU - Cooper, Jonathan B.

PY - 2021/8/1

Y1 - 2021/8/1

N2 - In many prokaryotes, the first step of threonine metabolism is catalysed by the enzyme threonine dehydrogenase (TDH), which uses NAD+ to oxidize its substrate to 2-amino-3-ketobutyrate. The absence of a functional TDH gene in humans suggests that inhibitors of this enzyme may have therapeutic potential against pathogens which are reliant on this enzyme. Here, TDH from Clostridium difficile has been cloned and overexpressed, and the X-ray structure of the apoenzyme form has been determined at 2.6 Å resolution.

AB - In many prokaryotes, the first step of threonine metabolism is catalysed by the enzyme threonine dehydrogenase (TDH), which uses NAD+ to oxidize its substrate to 2-amino-3-ketobutyrate. The absence of a functional TDH gene in humans suggests that inhibitors of this enzyme may have therapeutic potential against pathogens which are reliant on this enzyme. Here, TDH from Clostridium difficile has been cloned and overexpressed, and the X-ray structure of the apoenzyme form has been determined at 2.6 Å resolution.

KW - threonine dehydrogenase

KW - Clostridium difficile

KW - protein crystallography

KW - molecular replacement

KW - refinement

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JF - Acta Crystallographica Section F Structural Biology Communications

IS - 8

ER -

The X-ray structure of L-threonine dehydrogenase from the common hospital pathogen Clostridium difficile

Abstract

Keywords

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The X-ray structure of _L-threonine dehydrogenase from the common hospital pathogen Clostridium difficile