Transcutaneous anaesthetic nano-enabled hydrogels for eyelid surgery

Katerina Lalatsa; Priyanka V Patel; Yujiao Sun; Chong C Kiun; Ferishtah Karimi; Jurgita Zekonyte; Krisztina Emeriewen; George M. Saleh

doi:10.1016/j.ijpharm.2019.119003

Transcutaneous anaesthetic nano-enabled hydrogels for eyelid surgery

Katerina Lalatsa, Priyanka V Patel, Yujiao Sun, Chong C Kiun, Ferishtah Karimi, Jurgita Zekonyte, Krisztina Emeriewen, George M. Saleh

Moorfields Eye Hospital

Research output: Contribution to journal › Article › peer-review

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Abstract

Local anaesthetics are administered as a diffuse superficial slow injection in blepharoplasty. Current transcutaneous local anaesthetic formulations are not licensed for use on the face due to safety concerns. Here we report for the first time the permeation of local anaesthetics (lidocaine, bupivacaine loaded SNEDDS and their hydrogels across human eyelid and mouse skin as a novel and ocular safe formulation for eyelid surgery. SNEDDS were loaded with high levels of anaesthetics and incorporated within carbomer hydrogels to yield nano-enabled gels. Lidocaine hydrogels have a significantly reduced lag time compared to EMLA, while they enhance lidocaine flux across human eyelid skin by 5.2 fold. Ex vivo tape stripping experiments indicated localisation of anaesthetics within the stratum corneum and dermis. Initial histopathological studies have shown no apparent signs of skin irritation. These results highlight the potential clinical capability of nano-enabled anaesthetic hydrogels as a non-invasive anaesthetic procedure for eyelid surgery.

Original language	English
Article number	119003
Number of pages	9
Journal	International Journal of Pharmaceutics
Volume	577
Early online date	11 Jan 2020
DOIs	https://doi.org/10.1016/j.ijpharm.2019.119003
Publication status	Published - 15 Mar 2020

Keywords

Lidocaine
Bupivacaine
Anaesthetics
Self-nanoemulsifying drug delivery systems (SNEDDS)
hydrogels
eyelid
tape stripping
Franz cell diffusion assays

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10.1016/j.ijpharm.2019.119003

IJP-D-19-02157R1 PureAccepted author manuscript (Post-print), 688 KBLicence: CC BY-NC-ND

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title = "Transcutaneous anaesthetic nano-enabled hydrogels for eyelid surgery",

abstract = "Local anaesthetics are administered as a diffuse superficial slow injection in blepharoplasty. Current transcutaneous local anaesthetic formulations are not licensed for use on the face due to safety concerns. Here we report for the first time the permeation of local anaesthetics (lidocaine, bupivacaine loaded SNEDDS and their hydrogels across human eyelid and mouse skin as a novel and ocular safe formulation for eyelid surgery. SNEDDS were loaded with high levels of anaesthetics and incorporated within carbomer hydrogels to yield nano-enabled gels. Lidocaine hydrogels have a significantly reduced lag time compared to EMLA, while they enhance lidocaine flux across human eyelid skin by 5.2 fold. Ex vivo tape stripping experiments indicated localisation of anaesthetics within the stratum corneum and dermis. Initial histopathological studies have shown no apparent signs of skin irritation. These results highlight the potential clinical capability of nano-enabled anaesthetic hydrogels as a non-invasive anaesthetic procedure for eyelid surgery. ",

keywords = "Lidocaine, Bupivacaine, Anaesthetics, Self-nanoemulsifying drug delivery systems (SNEDDS), hydrogels, eyelid, tape stripping, Franz cell diffusion assays",

author = "Katerina Lalatsa and Patel, {Priyanka V} and Yujiao Sun and Kiun, {Chong C} and Ferishtah Karimi and Jurgita Zekonyte and Krisztina Emeriewen and Saleh, {George M.}",

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doi = "10.1016/j.ijpharm.2019.119003",

language = "English",

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AU - Lalatsa, Katerina

AU - Patel, Priyanka V

AU - Sun, Yujiao

AU - Kiun, Chong C

AU - Karimi, Ferishtah

AU - Zekonyte, Jurgita

AU - Emeriewen, Krisztina

AU - Saleh, George M.

PY - 2020/3/15

Y1 - 2020/3/15

N2 - Local anaesthetics are administered as a diffuse superficial slow injection in blepharoplasty. Current transcutaneous local anaesthetic formulations are not licensed for use on the face due to safety concerns. Here we report for the first time the permeation of local anaesthetics (lidocaine, bupivacaine loaded SNEDDS and their hydrogels across human eyelid and mouse skin as a novel and ocular safe formulation for eyelid surgery. SNEDDS were loaded with high levels of anaesthetics and incorporated within carbomer hydrogels to yield nano-enabled gels. Lidocaine hydrogels have a significantly reduced lag time compared to EMLA, while they enhance lidocaine flux across human eyelid skin by 5.2 fold. Ex vivo tape stripping experiments indicated localisation of anaesthetics within the stratum corneum and dermis. Initial histopathological studies have shown no apparent signs of skin irritation. These results highlight the potential clinical capability of nano-enabled anaesthetic hydrogels as a non-invasive anaesthetic procedure for eyelid surgery.

AB - Local anaesthetics are administered as a diffuse superficial slow injection in blepharoplasty. Current transcutaneous local anaesthetic formulations are not licensed for use on the face due to safety concerns. Here we report for the first time the permeation of local anaesthetics (lidocaine, bupivacaine loaded SNEDDS and their hydrogels across human eyelid and mouse skin as a novel and ocular safe formulation for eyelid surgery. SNEDDS were loaded with high levels of anaesthetics and incorporated within carbomer hydrogels to yield nano-enabled gels. Lidocaine hydrogels have a significantly reduced lag time compared to EMLA, while they enhance lidocaine flux across human eyelid skin by 5.2 fold. Ex vivo tape stripping experiments indicated localisation of anaesthetics within the stratum corneum and dermis. Initial histopathological studies have shown no apparent signs of skin irritation. These results highlight the potential clinical capability of nano-enabled anaesthetic hydrogels as a non-invasive anaesthetic procedure for eyelid surgery.

KW - Lidocaine

KW - Bupivacaine

KW - Anaesthetics

KW - Self-nanoemulsifying drug delivery systems (SNEDDS)

KW - hydrogels

KW - eyelid

KW - tape stripping

KW - Franz cell diffusion assays

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DO - 10.1016/j.ijpharm.2019.119003

M3 - Article

SN - 0378-5173

VL - 577

JO - International Journal of Pharmaceutics

JF - International Journal of Pharmaceutics

M1 - 119003

ER -

Transcutaneous anaesthetic nano-enabled hydrogels for eyelid surgery

Abstract

Keywords

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