@inbook{bf43b04982414d2e8ae31e9eeaf76f01,
title = "Transient expression in HEK 293 cells: an alternative to E. coli for the production of secreted and intracellular mammalian proteins",
abstract = "Transient transfection of human embryonic kidney cells (HEK 293) enables the rapid and affordable lab-scale production of recombinant proteins. In this chapter protocols for the expression and purification of both secreted and intracellular proteins using transient expression in HEK 293 cells are described.",
author = "Nettleship, {Joanne E.} and Watson, {Peter J.} and Nahid Rahman-Huq and Louise Fairall and Posner, {Mareike G.} and Abhishek Upadhyay and Yamini Reddivari and Chamberlain, {Jonathan M. G.} and Kolstoe, {Simon E.} and Stefan Bagby and Schwabe, {John W. R.} and Owens, {Raymond J.}",
year = "2015",
doi = "10.1007/978-1-4939-2205-5_11",
language = "English",
isbn = "978-1493922048",
series = "Methods in Molecular Biology",
publisher = "Springer",
pages = "209--222",
editor = "Elena Garc{\'i}a-Fruit{\'o}s",
booktitle = "Insoluble Proteins",
}