Transient expression in HEK 293 cells: an alternative to E. coli for the production of secreted and intracellular mammalian proteins

Joanne E. Nettleship, Peter J. Watson, Nahid Rahman-Huq, Louise Fairall, Mareike G. Posner, Abhishek Upadhyay, Yamini Reddivari, Jonathan M. G. Chamberlain, Simon E. Kolstoe, Stefan Bagby, John W. R. Schwabe, Raymond J. Owens

Research output: Chapter in Book/Report/Conference proceedingChapter (peer-reviewed)peer-review

Abstract

Transient transfection of human embryonic kidney cells (HEK 293) enables the rapid and affordable lab-scale production of recombinant proteins. In this chapter protocols for the expression and purification of both secreted and intracellular proteins using transient expression in HEK 293 cells are described.

Original languageEnglish
Title of host publicationInsoluble Proteins
Subtitle of host publicationMethods and Protocols
EditorsElena García-Fruitós
Place of PublicationNew York
PublisherSpringer
Pages209-222
Number of pages14
ISBN (Electronic)978-1493922055
ISBN (Print)978-1493922048
DOIs
Publication statusPublished - 2015

Publication series

NameMethods in Molecular Biology
PublisherSpringer
Volume1258
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Fingerprint

Dive into the research topics of 'Transient expression in HEK 293 cells: an alternative to E. coli for the production of secreted and intracellular mammalian proteins'. Together they form a unique fingerprint.

Cite this