Transient expression in HEK 293 cells: an alternative to E. coli for the production of secreted and intracellular mammalian proteins

Joanne E. Nettleship; Peter J. Watson; Nahid Rahman-Huq; Louise Fairall; Mareike G. Posner; Abhishek Upadhyay; Yamini Reddivari; Jonathan M. G. Chamberlain; Simon E. Kolstoe; Stefan Bagby; John W. R. Schwabe; Raymond J. Owens

doi:10.1007/978-1-4939-2205-5_11

Transient expression in HEK 293 cells: an alternative to E. coli for the production of secreted and intracellular mammalian proteins

Joanne E. Nettleship, Peter J. Watson, Nahid Rahman-Huq, Louise Fairall, Mareike G. Posner, Abhishek Upadhyay, Yamini Reddivari, Jonathan M. G. Chamberlain, Simon E. Kolstoe, Stefan Bagby, John W. R. Schwabe, Raymond J. Owens

Institute of Life Sciences and Healthcare

Oxford Protein Production Facility-UK

Research output: Chapter in Book/Report/Conference proceeding › Chapter (peer-reviewed) › peer-review

Abstract

Transient transfection of human embryonic kidney cells (HEK 293) enables the rapid and affordable lab-scale production of recombinant proteins. In this chapter protocols for the expression and purification of both secreted and intracellular proteins using transient expression in HEK 293 cells are described.

Original language	English
Title of host publication	Insoluble Proteins
Subtitle of host publication	Methods and Protocols
Editors	Elena García-Fruitós
Place of Publication	New York
Publisher	Springer
Pages	209-222
Number of pages	14
ISBN (Electronic)	978-1493922055
ISBN (Print)	978-1493922048
DOIs	https://doi.org/10.1007/978-1-4939-2205-5_11
Publication status	Published - 2015

Publication series

Name	Methods in Molecular Biology
Publisher	Springer
Volume	1258
ISSN (Print)	1064-3745
ISSN (Electronic)	1940-6029

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10.1007/978-1-4939-2205-5_11

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Nettleship, J. E., Watson, P. J., Rahman-Huq, N., Fairall, L., Posner, M. G., Upadhyay, A., Reddivari, Y., Chamberlain, J. M. G., Kolstoe, S. E., Bagby, S., Schwabe, J. W. R., & Owens, R. J. (2015). Transient expression in HEK 293 cells: an alternative to E. coli for the production of secreted and intracellular mammalian proteins. In E. García-Fruitós (Ed.), Insoluble Proteins: Methods and Protocols (pp. 209-222). (Methods in Molecular Biology; Vol. 1258). Springer. https://doi.org/10.1007/978-1-4939-2205-5_11

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title = "Transient expression in HEK 293 cells: an alternative to E. coli for the production of secreted and intracellular mammalian proteins",

abstract = "Transient transfection of human embryonic kidney cells (HEK 293) enables the rapid and affordable lab-scale production of recombinant proteins. In this chapter protocols for the expression and purification of both secreted and intracellular proteins using transient expression in HEK 293 cells are described.",

author = "Nettleship, \{Joanne E.\} and Watson, \{Peter J.\} and Nahid Rahman-Huq and Louise Fairall and Posner, \{Mareike G.\} and Abhishek Upadhyay and Yamini Reddivari and Chamberlain, \{Jonathan M. G.\} and Kolstoe, \{Simon E.\} and Stefan Bagby and Schwabe, \{John W. R.\} and Owens, \{Raymond J.\}",

year = "2015",

doi = "10.1007/978-1-4939-2205-5\_11",

language = "English",

isbn = "978-1493922048",

series = "Methods in Molecular Biology",

publisher = "Springer",

pages = "209--222",

editor = "Elena Garc{\'i}a-Fruit{\'o}s",

booktitle = "Insoluble Proteins",

address = "Netherlands",

}

Nettleship, JE, Watson, PJ, Rahman-Huq, N, Fairall, L, Posner, MG, Upadhyay, A, Reddivari, Y, Chamberlain, JMG, Kolstoe, SE, Bagby, S, Schwabe, JWR & Owens, RJ 2015, Transient expression in HEK 293 cells: an alternative to E. coli for the production of secreted and intracellular mammalian proteins. in E García-Fruitós (ed.), Insoluble Proteins: Methods and Protocols. Methods in Molecular Biology, vol. 1258, Springer, New York, pp. 209-222. https://doi.org/10.1007/978-1-4939-2205-5_11

Transient expression in HEK 293 cells: an alternative to E. coli for the production of secreted and intracellular mammalian proteins. / Nettleship, Joanne E.; Watson, Peter J.; Rahman-Huq, Nahid et al.
Insoluble Proteins: Methods and Protocols. ed. / Elena García-Fruitós. New York: Springer, 2015. p. 209-222 (Methods in Molecular Biology; Vol. 1258).

Research output: Chapter in Book/Report/Conference proceeding › Chapter (peer-reviewed) › peer-review

TY - CHAP

T1 - Transient expression in HEK 293 cells

T2 - an alternative to E. coli for the production of secreted and intracellular mammalian proteins

AU - Nettleship, Joanne E.

AU - Watson, Peter J.

AU - Rahman-Huq, Nahid

AU - Fairall, Louise

AU - Posner, Mareike G.

AU - Upadhyay, Abhishek

AU - Reddivari, Yamini

AU - Chamberlain, Jonathan M. G.

AU - Kolstoe, Simon E.

AU - Bagby, Stefan

AU - Schwabe, John W. R.

AU - Owens, Raymond J.

PY - 2015

Y1 - 2015

N2 - Transient transfection of human embryonic kidney cells (HEK 293) enables the rapid and affordable lab-scale production of recombinant proteins. In this chapter protocols for the expression and purification of both secreted and intracellular proteins using transient expression in HEK 293 cells are described.

AB - Transient transfection of human embryonic kidney cells (HEK 293) enables the rapid and affordable lab-scale production of recombinant proteins. In this chapter protocols for the expression and purification of both secreted and intracellular proteins using transient expression in HEK 293 cells are described.

U2 - 10.1007/978-1-4939-2205-5_11

DO - 10.1007/978-1-4939-2205-5_11

M3 - Chapter (peer-reviewed)

C2 - 25447866

SN - 978-1493922048

T3 - Methods in Molecular Biology

SP - 209

EP - 222

BT - Insoluble Proteins

A2 - García-Fruitós, Elena

PB - Springer

CY - New York

ER -

Nettleship JE, Watson PJ, Rahman-Huq N, Fairall L, Posner MG, Upadhyay A et al. Transient expression in HEK 293 cells: an alternative to E. coli for the production of secreted and intracellular mammalian proteins. In García-Fruitós E, editor, Insoluble Proteins: Methods and Protocols. New York: Springer. 2015. p. 209-222. (Methods in Molecular Biology). Epub 2014 Nov 16. doi: 10.1007/978-1-4939-2205-5_11

Transient expression in HEK 293 cells: an alternative to E. coli for the production of secreted and intracellular mammalian proteins

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