Two are better than one

S. Halford; Darren Gowers; R. Sessions

doi:10.1038/78911

Two are better than one

S. Halford, Darren Gowers, R. Sessions

School of Biological Sciences

Research output: Contribution to journal › Article › peer-review

Abstract

A crystal structure of a tetrameric restriction enzyme, NgoMIV, bound to two DNA duplexes has been determined. Two subunits contact each duplex in much the same manner as a dimeric restriction enzyme recognizing a single site, but the dimeric units are packed back-to-back, placing the duplexes on opposite sides of the tetramer. Interaction with two recognition sites, presumably via looping, enhances NgoMIV activity.

Original language	English
Pages (from-to)	705-707
Number of pages	3
Journal	Nature Structural Biology
Volume	7
Issue number	9
DOIs	https://doi.org/10.1038/78911
Publication status	Published - 2000

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10.1038/78911

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title = "Two are better than one",

abstract = "A crystal structure of a tetrameric restriction enzyme, NgoMIV, bound to two DNA duplexes has been determined. Two subunits contact each duplex in much the same manner as a dimeric restriction enzyme recognizing a single site, but the dimeric units are packed back-to-back, placing the duplexes on opposite sides of the tetramer. Interaction with two recognition sites, presumably via looping, enhances NgoMIV activity.",

author = "S. Halford and Darren Gowers and R. Sessions",

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AU - Halford, S.

AU - Gowers, Darren

AU - Sessions, R.

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AB - A crystal structure of a tetrameric restriction enzyme, NgoMIV, bound to two DNA duplexes has been determined. Two subunits contact each duplex in much the same manner as a dimeric restriction enzyme recognizing a single site, but the dimeric units are packed back-to-back, placing the duplexes on opposite sides of the tetramer. Interaction with two recognition sites, presumably via looping, enhances NgoMIV activity.

U2 - 10.1038/78911

DO - 10.1038/78911

M3 - Article

SN - 1545-9993

VL - 7

SP - 705

EP - 707

JO - Nature Structural Biology

JF - Nature Structural Biology

IS - 9

ER -

Two are better than one

Abstract

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