Visualisation of plasmid DNA molecules containing T15A15 repeat sequences by atomic force microscopy

P. C. Zhang; J. E. Mitchell; J. R. Smith; J. A. McClellan; S. A. Campbell; S. F. Newbury

Visualisation of plasmid DNA molecules containing T₁₅A₁₅ repeat sequences by atomic force microscopy

P. C. Zhang, J. E. Mitchell, J. R. Smith, J. A. McClellan, S. A. Campbell, S. F. Newbury^*

^*Corresponding author for this work

University of Oxford

Research output: Contribution to journal › Article › peer-review

Abstract

We have used atomic force microscopy to examine the conformations of a DNA plasmid which contains a repeated sequence that is able to form an unusual DNA structure. About 16.8% of plasmid molecules containing the sequence (T₁₅A₁₅)₂ adopted a compacted rod-shaped form compared to 89.5% of control molecules without the repeated sequence. The secondary structure- forming sequence therefore changed the conformation of the supercoiled plasmid in the predicted manner. In about 40.2% of plasmids with the (T₁₅A₁₅)₂ sequence a short, branched structure was visible. We speculate that this object may represent a non-standard local DNA conformation, and suggest an interpretation of the structure.

Original language	English
Pages (from-to)	303-311
Number of pages	9
Journal	Probe Microscopy
Volume	1
Issue number	4
Publication status	Published - 19 Oct 1999

Keywords

Atomic force microscopy
Cruciforms
DNA structure
Supercoiling Repeated sequences

Access to Document

https://www.tandfonline.com/loi/gprm20

Cite this

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title = "Visualisation of plasmid DNA molecules containing T15A15 repeat sequences by atomic force microscopy",

abstract = "We have used atomic force microscopy to examine the conformations of a DNA plasmid which contains a repeated sequence that is able to form an unusual DNA structure. About 16.8% of plasmid molecules containing the sequence (T15A15)2 adopted a compacted rod-shaped form compared to 89.5% of control molecules without the repeated sequence. The secondary structure- forming sequence therefore changed the conformation of the supercoiled plasmid in the predicted manner. In about 40.2% of plasmids with the (T15A15)2 sequence a short, branched structure was visible. We speculate that this object may represent a non-standard local DNA conformation, and suggest an interpretation of the structure.",

keywords = "Atomic force microscopy, Cruciforms, DNA structure, Supercoiling Repeated sequences",

author = "Zhang, {P. C.} and Mitchell, {J. E.} and Smith, {J. R.} and McClellan, {J. A.} and Campbell, {S. A.} and Newbury, {S. F.}",

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T1 - Visualisation of plasmid DNA molecules containing T15A15 repeat sequences by atomic force microscopy

AU - Zhang, P. C.

AU - Mitchell, J. E.

AU - Smith, J. R.

AU - McClellan, J. A.

AU - Campbell, S. A.

AU - Newbury, S. F.

PY - 1999/10/19

Y1 - 1999/10/19

N2 - We have used atomic force microscopy to examine the conformations of a DNA plasmid which contains a repeated sequence that is able to form an unusual DNA structure. About 16.8% of plasmid molecules containing the sequence (T15A15)2 adopted a compacted rod-shaped form compared to 89.5% of control molecules without the repeated sequence. The secondary structure- forming sequence therefore changed the conformation of the supercoiled plasmid in the predicted manner. In about 40.2% of plasmids with the (T15A15)2 sequence a short, branched structure was visible. We speculate that this object may represent a non-standard local DNA conformation, and suggest an interpretation of the structure.

AB - We have used atomic force microscopy to examine the conformations of a DNA plasmid which contains a repeated sequence that is able to form an unusual DNA structure. About 16.8% of plasmid molecules containing the sequence (T15A15)2 adopted a compacted rod-shaped form compared to 89.5% of control molecules without the repeated sequence. The secondary structure- forming sequence therefore changed the conformation of the supercoiled plasmid in the predicted manner. In about 40.2% of plasmids with the (T15A15)2 sequence a short, branched structure was visible. We speculate that this object may represent a non-standard local DNA conformation, and suggest an interpretation of the structure.

KW - Atomic force microscopy

KW - Cruciforms

KW - DNA structure

KW - Supercoiling Repeated sequences

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