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Supplementary material for 'Production and characterization of glycolipid biosurfactant from Achromobacter sp. (PS1) isolate using one-factor-at-a-time (OFAT) approach with feasible utilization of ammonia-soaked lignocellulosic pretreated residues'.

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A copy of the supplementary material will be available here (along with the article post-print) once the embargo period has ended (26/04/2020).

If your organisation has a subscription for the journal publisher (sometimes termed ‘authorised user’ or similar), the supplementary files are available online at https://doi.org/10.1007/s00449-019-02128-3


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Fig. S1: Characterization of the biosurfactant produced by Achromobacter sp. (PS1); LC-MS spectra obtained in positive ion mode of the rhamnolipid sample

Fig. S2: NMR spectrum of mono-rhamnolipid biosurfactant produced from Achromobacter sp. (PS1) (a) 1H NMR spectrum. (b) HSQC spectrum showing chemical shifts of 1H (horizontal axis) and 13C (vertical axis). The single anomeric signals at δ 4.91/94.18 suggest a β-L-Rha(1ʹ↔1)-hydroxy-fatty acid linkages of mono-rhamnolipid

Fig. S3: NMR spectrum of di-rhamnolipid biosurfactant produced from Achromobacter sp. (PS1) (a) 1H NMR spectrum (b) HSQC spectrum showing chemical shifts of 1H (horizontal axis) and 13C (vertical axis). The anomeric signals at δ 4.91/94.18 and 4.90/102.40 suggest a β-L-Rha(1ʹ↔1)-hydroxyfatty acid and β-L-Rha (1ʺ↔2 ʹ)- β-L-Rha linkages of di-rhamnolipid respectively

Fig. S4. (a) Effect of varying concentrations of FeSO4 (b) Effect of initial phosphate concentration (mM) on rhamnolipid production.

Fig. S5: Effect of (a) temperature stability at (i) (30-90°C) after 24 Hrs (ii) dotted line (100°C) and straight line (121°C) till 120 minutes (b) pH stability (at 48 hr) and (c) saline stability (at 48 hr) of biosurfactant produced from Achromobacter sp. (PS1).
*Residual activity 100 % of control corresponds to E24 of 63.78 %

Table 1. Identification of [M + Na] + and [M + K] + ions observed in the LC-MS/MS spectra corresponding to mono and di-rhamnolipids present in the partially purified biosurfactant

Table 2. Total (%) composition of raw lignocellulosic residues

Table 3. Total (%) composition of sugars released in hydrolysates of the pretreated and enzyme saccharified lignocellulosic residues
Date made available26 Apr 2019
PublisherSpringer Nature

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