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Electron Microscope Unit (EMU) - Including Transmission Electron Microscope (JEOL 1400) and Scanning Electron Microscopy JEOL 6060LV

Facility/Equipment: Equipment

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Description

The School of Biological Sciences runs an Electron Microscope Unit (EMU) in which specimens are prepared and imaged by transmission electron microscopy (TEM) and scanning electron microscopy (SEM).

The TEM is a JEOL 1400 capable of operating at 120kV acceleration voltage, with a goniometer stage and a base-mounted AMT digital camera. Sectioning for the TEM uses the super sharpness of glass knives (Leica KMR3 glass knife maker) operating on a Leica Ultracut 7 ultratome.

The SEM is a JEOL 6060LV capable of imaging in secondary and backscattered electron mode. It has an Oxford Instruments 80mm X-Max EDS probe
that provides data on elemental composition and microscopic distribution. Delicate specimens are prepared for imaging in the vacuum within the SEM using a Leica CPD 300 critical point dryer then coated with a conducting layer using a Quorum Q150 sputter and carbon coater.

The TEM and SEM have be used to examine a very wide range of specimens, including novel drug delivery mechanisms (TETFUND, Nigeria studentship), gliomas (FCT Portugal plus Brain Tumour Research), fiddler crabs (Operation Wallacea), microfossils, archaeological specimens (Mary Rose Trust), wood borers (BBSRC and Leverhulme Trust funding) and animals affected by pollution (NERC funding).

The EMU equipment is supported by a specialist technician and an academic staff member with extensive electron microscopy experience.

Available for loan

Yes -

ID: 13676184