Skip to content

A direct-staining method to evaluate the mucoadhesion of polymers from aqueous dispersion

Research output: Contribution to journalArticle

A novel technique to evaluate polymer adhesion to human buccal cells following exposure to aqueous polymer dispersion, both in vitro and in vivo, is described. Adhering polymer has been visualised by staining with 0.1% (w/v) of either Alcian blue (60 min) or Eosin (10 min) solution, uncomplexed dye being removed by 0.25 M sucrose washings. The extent of polymer adhesion was quantified by measuring the relative staining intensity of control and polymer-treated cells by image analysis. In vitro, Carbopol 974P, polycarbophil (Noveon AA-1) and chitosan (CL 113) were found to adhere to human buccal cells from 0.10% (w/w) aqueous dispersions of these polymers. Following in vivo administration as a mouthwash, these polymers persisted upon the human buccal mucosa for at least 1 h.

Original languageEnglish
Pages (from-to)1-6
Number of pages6
JournalJournal of Controlled Release
Issue number1-2
Publication statusPublished - 9 Nov 2001

Related information

Relations Get citation (various referencing formats)

ID: 11420252