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A lncRNA fine tunes the dynamics of a cell state transition involving Lin28, let-7 and de novo DNA methylation

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A lncRNA fine tunes the dynamics of a cell state transition involving Lin28, let-7 and de novo DNA methylation. / Li, Meng Amy; Amaral, Paulo P.; Cheung, Priscilla; Bergmann, Jan H.; Kinoshita, Masaki; Kalkan, Tüzer; Ralser, Meryem; Robson, Sam; Meyenn, Ferdinand von; Paramor, Maike; Yang, Fengtang; Chen, Caifu; Nichols, Jennifer; Spector, David L.; Kouzarides, Tony; He, Lin; Smith, Austin.

In: eLife, Vol. 6, e23468, 18.08.2017.

Research output: Contribution to journalArticlepeer-review

Harvard

Li, MA, Amaral, PP, Cheung, P, Bergmann, JH, Kinoshita, M, Kalkan, T, Ralser, M, Robson, S, Meyenn, FV, Paramor, M, Yang, F, Chen, C, Nichols, J, Spector, DL, Kouzarides, T, He, L & Smith, A 2017, 'A lncRNA fine tunes the dynamics of a cell state transition involving Lin28, let-7 and de novo DNA methylation', eLife, vol. 6, e23468. https://doi.org/10.7554/eLife.23468

APA

Li, M. A., Amaral, P. P., Cheung, P., Bergmann, J. H., Kinoshita, M., Kalkan, T., Ralser, M., Robson, S., Meyenn, F. V., Paramor, M., Yang, F., Chen, C., Nichols, J., Spector, D. L., Kouzarides, T., He, L., & Smith, A. (2017). A lncRNA fine tunes the dynamics of a cell state transition involving Lin28, let-7 and de novo DNA methylation. eLife, 6, [e23468]. https://doi.org/10.7554/eLife.23468

Vancouver

Li MA, Amaral PP, Cheung P, Bergmann JH, Kinoshita M, Kalkan T et al. A lncRNA fine tunes the dynamics of a cell state transition involving Lin28, let-7 and de novo DNA methylation. eLife. 2017 Aug 18;6. e23468. https://doi.org/10.7554/eLife.23468

Author

Li, Meng Amy ; Amaral, Paulo P. ; Cheung, Priscilla ; Bergmann, Jan H. ; Kinoshita, Masaki ; Kalkan, Tüzer ; Ralser, Meryem ; Robson, Sam ; Meyenn, Ferdinand von ; Paramor, Maike ; Yang, Fengtang ; Chen, Caifu ; Nichols, Jennifer ; Spector, David L. ; Kouzarides, Tony ; He, Lin ; Smith, Austin. / A lncRNA fine tunes the dynamics of a cell state transition involving Lin28, let-7 and de novo DNA methylation. In: eLife. 2017 ; Vol. 6.

Bibtex

@article{c0c7f155400144daa41ffba3e021b068,
title = "A lncRNA fine tunes the dynamics of a cell state transition involving Lin28, let-7 and de novo DNA methylation",
abstract = "Execution of pluripotency requires progression from the na{\"i}ve status represented by mouse embryonic stem cells (ESCs) to a state capacitated for lineage specification. This transition is coordinated at multiple levels. Non-coding RNAs may contribute to this regulatory orchestra. We identified a rodent-specific long non-coding RNA (lncRNA) linc1281, hereafter Ephemeron (Eprn), that modulates the dynamics of exit from na{\"i}ve pluripotency. Eprn deletion delays the extinction of ESC identity, an effect associated with perduring Nanog expression. In the absence of Eprn, Lin28a expression is reduced which results in persistence of let-7 microRNAs, and the up-regulation of de novo methyltransferases Dnmt3a/b is delayed. Dnmt3a/b deletion retards ES cell transition, correlating with delayed Nanog promoter methylation and phenocopying loss of Eprn or Lin28a. The connection from lncRNA to miRNA and DNA methylation facilitates the acute extinction of na{\"i}ve pluripotency, a pre-requisite for rapid progression from preimplantation epiblast to gastrulation in rodents. Eprn illustrates how lncRNAs may introduce species-specific network modulations.",
keywords = "Journal Article, RCUK, BBSRC, BB/M004023/1, MRC, G1100526/1",
author = "Li, {Meng Amy} and Amaral, {Paulo P.} and Priscilla Cheung and Bergmann, {Jan H.} and Masaki Kinoshita and T{\"u}zer Kalkan and Meryem Ralser and Sam Robson and Meyenn, {Ferdinand von} and Maike Paramor and Fengtang Yang and Caifu Chen and Jennifer Nichols and Spector, {David L.} and Tony Kouzarides and Lin He and Austin Smith",
year = "2017",
month = aug,
day = "18",
doi = "10.7554/eLife.23468",
language = "English",
volume = "6",
journal = "eLife",
issn = "2050-084X",
publisher = "eLife Sciences Publication",

}

RIS

TY - JOUR

T1 - A lncRNA fine tunes the dynamics of a cell state transition involving Lin28, let-7 and de novo DNA methylation

AU - Li, Meng Amy

AU - Amaral, Paulo P.

AU - Cheung, Priscilla

AU - Bergmann, Jan H.

AU - Kinoshita, Masaki

AU - Kalkan, Tüzer

AU - Ralser, Meryem

AU - Robson, Sam

AU - Meyenn, Ferdinand von

AU - Paramor, Maike

AU - Yang, Fengtang

AU - Chen, Caifu

AU - Nichols, Jennifer

AU - Spector, David L.

AU - Kouzarides, Tony

AU - He, Lin

AU - Smith, Austin

PY - 2017/8/18

Y1 - 2017/8/18

N2 - Execution of pluripotency requires progression from the naïve status represented by mouse embryonic stem cells (ESCs) to a state capacitated for lineage specification. This transition is coordinated at multiple levels. Non-coding RNAs may contribute to this regulatory orchestra. We identified a rodent-specific long non-coding RNA (lncRNA) linc1281, hereafter Ephemeron (Eprn), that modulates the dynamics of exit from naïve pluripotency. Eprn deletion delays the extinction of ESC identity, an effect associated with perduring Nanog expression. In the absence of Eprn, Lin28a expression is reduced which results in persistence of let-7 microRNAs, and the up-regulation of de novo methyltransferases Dnmt3a/b is delayed. Dnmt3a/b deletion retards ES cell transition, correlating with delayed Nanog promoter methylation and phenocopying loss of Eprn or Lin28a. The connection from lncRNA to miRNA and DNA methylation facilitates the acute extinction of naïve pluripotency, a pre-requisite for rapid progression from preimplantation epiblast to gastrulation in rodents. Eprn illustrates how lncRNAs may introduce species-specific network modulations.

AB - Execution of pluripotency requires progression from the naïve status represented by mouse embryonic stem cells (ESCs) to a state capacitated for lineage specification. This transition is coordinated at multiple levels. Non-coding RNAs may contribute to this regulatory orchestra. We identified a rodent-specific long non-coding RNA (lncRNA) linc1281, hereafter Ephemeron (Eprn), that modulates the dynamics of exit from naïve pluripotency. Eprn deletion delays the extinction of ESC identity, an effect associated with perduring Nanog expression. In the absence of Eprn, Lin28a expression is reduced which results in persistence of let-7 microRNAs, and the up-regulation of de novo methyltransferases Dnmt3a/b is delayed. Dnmt3a/b deletion retards ES cell transition, correlating with delayed Nanog promoter methylation and phenocopying loss of Eprn or Lin28a. The connection from lncRNA to miRNA and DNA methylation facilitates the acute extinction of naïve pluripotency, a pre-requisite for rapid progression from preimplantation epiblast to gastrulation in rodents. Eprn illustrates how lncRNAs may introduce species-specific network modulations.

KW - Journal Article

KW - RCUK

KW - BBSRC

KW - BB/M004023/1

KW - MRC

KW - G1100526/1

U2 - 10.7554/eLife.23468

DO - 10.7554/eLife.23468

M3 - Article

C2 - 28820723

VL - 6

JO - eLife

JF - eLife

SN - 2050-084X

M1 - e23468

ER -

ID: 7656190