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A practicable laboratory flow-through exposure system for assessing the health effects of effluents in fish

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  • Karen Thorpe
  • R. Benstead
  • P. Eccles
  • G. Maack
  • T. Williams
  • C. Tyler
The knowledge that exposure to estrogenic wastewater treatment work (WwTW) effluents induces a range of reproductive abnormalities in fish has highlighted the need to understand the wider health effects of effluents. Access to laboratory-based testing systems for WwTW effluents could greatly facilitate this endeavour. In this investigation, a laboratory-based test system was developed and applied for WwTW effluents using fathead minnows (Pimephales promelas). Sexually maturing fathead minnows were exposed, under flow-through conditions in the laboratory, for up to 21 days to graded concentrations of effluent from three different UK (temperate) WwTWs. The stability of the estrogenic component within the test system was assessed via measurements for estradiol and estrone concentrations in the effluent, and through determining estrogenic responses in an in vitro recombinant yeast estrogen screen (rYES) and in fish (plasma vitellogenin induction). The estrogen component of the effluents was stable within the holding system used (chilled
Original languageEnglish
Pages (from-to)164-172
Number of pages9
JournalAquatic Toxicology
Issue number3
Publication statusPublished - 7 Jul 2008

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