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Aberrant nucleo-cytoplasmic cross-talk results in donor cell mtDNA persistence in cloned embryos

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Aberrant nucleo-cytoplasmic cross-talk results in donor cell mtDNA persistence in cloned embryos. / Lloyd, Rhiannon; Lee, J.; Alberio, R.; Bowles, E.; Ramalho-Santos, J.; Campbell, K.; St John, J.

In: Genetics, Vol. 172, No. 4, 04.2006, p. 2515-2527.

Research output: Contribution to journalArticlepeer-review

Harvard

Lloyd, R, Lee, J, Alberio, R, Bowles, E, Ramalho-Santos, J, Campbell, K & St John, J 2006, 'Aberrant nucleo-cytoplasmic cross-talk results in donor cell mtDNA persistence in cloned embryos', Genetics, vol. 172, no. 4, pp. 2515-2527. https://doi.org/10.1534/genetics.105.055145

APA

Lloyd, R., Lee, J., Alberio, R., Bowles, E., Ramalho-Santos, J., Campbell, K., & St John, J. (2006). Aberrant nucleo-cytoplasmic cross-talk results in donor cell mtDNA persistence in cloned embryos. Genetics, 172(4), 2515-2527. https://doi.org/10.1534/genetics.105.055145

Vancouver

Lloyd R, Lee J, Alberio R, Bowles E, Ramalho-Santos J, Campbell K et al. Aberrant nucleo-cytoplasmic cross-talk results in donor cell mtDNA persistence in cloned embryos. Genetics. 2006 Apr;172(4):2515-2527. https://doi.org/10.1534/genetics.105.055145

Author

Lloyd, Rhiannon ; Lee, J. ; Alberio, R. ; Bowles, E. ; Ramalho-Santos, J. ; Campbell, K. ; St John, J. / Aberrant nucleo-cytoplasmic cross-talk results in donor cell mtDNA persistence in cloned embryos. In: Genetics. 2006 ; Vol. 172, No. 4. pp. 2515-2527.

Bibtex

@article{ae2924e34d4447648893a3a33748888b,
title = "Aberrant nucleo-cytoplasmic cross-talk results in donor cell mtDNA persistence in cloned embryos",
abstract = "Mitochondrial DNA is an extranuclear genome normally maternally inherited through the oocyte. However, the use of nuclear transfer can result in both donor cell and recipient oocyte mitochondrial DNA persisting through to blastocyst and being transmitted to the offspring. The degree of donor mitochondrial DNA transmission appears to be random and currently no evidence exists to explain this phenomenon. To determine whether this is a dilution factor or directly related to the transcriptional status of the donor cell in respect of mitochondrial DNA transcription factors, we have generated sheep nuclear transfer embryos using donor cells: (1) possessing their full mitochondrial DNA complement, (2) those partially depleted, and (3) those depleted but containing residual levels. For each donor type, donor mitochondrial DNA persisted in some blastocysts. It is evident from the donor cells used that nuclear-encoded mitochondrial DNA transcription and replication factors persist even after mitochondrial DNA depletion, as do transcripts for some of the mitochondrial-encoded genes. These cells are therefore still programmed to drive mitochondrial DNA replication and transcription. In nuclear transfer-derived embryos, we have observed the persistence of these nuclear-encoded mitochondrial DNA transcription and replication factors but not in those embryos generated through in vitro fertilization. Consequently, nucleo-mitochondrial interaction following nuclear transfer is out of sequence as the onset of mitochondrial replication is a postimplantation event.",
author = "Rhiannon Lloyd and J. Lee and R. Alberio and E. Bowles and J. Ramalho-Santos and K. Campbell and {St John}, J.",
year = "2006",
month = apr,
doi = "10.1534/genetics.105.055145",
language = "English",
volume = "172",
pages = "2515--2527",
journal = "Genetics",
issn = "0016-6731",
publisher = "Genetics Society of America",
number = "4",

}

RIS

TY - JOUR

T1 - Aberrant nucleo-cytoplasmic cross-talk results in donor cell mtDNA persistence in cloned embryos

AU - Lloyd, Rhiannon

AU - Lee, J.

AU - Alberio, R.

AU - Bowles, E.

AU - Ramalho-Santos, J.

AU - Campbell, K.

AU - St John, J.

PY - 2006/4

Y1 - 2006/4

N2 - Mitochondrial DNA is an extranuclear genome normally maternally inherited through the oocyte. However, the use of nuclear transfer can result in both donor cell and recipient oocyte mitochondrial DNA persisting through to blastocyst and being transmitted to the offspring. The degree of donor mitochondrial DNA transmission appears to be random and currently no evidence exists to explain this phenomenon. To determine whether this is a dilution factor or directly related to the transcriptional status of the donor cell in respect of mitochondrial DNA transcription factors, we have generated sheep nuclear transfer embryos using donor cells: (1) possessing their full mitochondrial DNA complement, (2) those partially depleted, and (3) those depleted but containing residual levels. For each donor type, donor mitochondrial DNA persisted in some blastocysts. It is evident from the donor cells used that nuclear-encoded mitochondrial DNA transcription and replication factors persist even after mitochondrial DNA depletion, as do transcripts for some of the mitochondrial-encoded genes. These cells are therefore still programmed to drive mitochondrial DNA replication and transcription. In nuclear transfer-derived embryos, we have observed the persistence of these nuclear-encoded mitochondrial DNA transcription and replication factors but not in those embryos generated through in vitro fertilization. Consequently, nucleo-mitochondrial interaction following nuclear transfer is out of sequence as the onset of mitochondrial replication is a postimplantation event.

AB - Mitochondrial DNA is an extranuclear genome normally maternally inherited through the oocyte. However, the use of nuclear transfer can result in both donor cell and recipient oocyte mitochondrial DNA persisting through to blastocyst and being transmitted to the offspring. The degree of donor mitochondrial DNA transmission appears to be random and currently no evidence exists to explain this phenomenon. To determine whether this is a dilution factor or directly related to the transcriptional status of the donor cell in respect of mitochondrial DNA transcription factors, we have generated sheep nuclear transfer embryos using donor cells: (1) possessing their full mitochondrial DNA complement, (2) those partially depleted, and (3) those depleted but containing residual levels. For each donor type, donor mitochondrial DNA persisted in some blastocysts. It is evident from the donor cells used that nuclear-encoded mitochondrial DNA transcription and replication factors persist even after mitochondrial DNA depletion, as do transcripts for some of the mitochondrial-encoded genes. These cells are therefore still programmed to drive mitochondrial DNA replication and transcription. In nuclear transfer-derived embryos, we have observed the persistence of these nuclear-encoded mitochondrial DNA transcription and replication factors but not in those embryos generated through in vitro fertilization. Consequently, nucleo-mitochondrial interaction following nuclear transfer is out of sequence as the onset of mitochondrial replication is a postimplantation event.

U2 - 10.1534/genetics.105.055145

DO - 10.1534/genetics.105.055145

M3 - Article

VL - 172

SP - 2515

EP - 2527

JO - Genetics

JF - Genetics

SN - 0016-6731

IS - 4

ER -

ID: 182590