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Altered colonic mucosal availability of n-3 and n-6 polyunsaturated fatty acids in ulcerative colitis and the relationship to disease activity

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Altered colonic mucosal availability of n-3 and n-6 polyunsaturated fatty acids in ulcerative colitis and the relationship to disease activity. / Pearl, Daniel S.; Masoodi, Mojgan; Eiden, Michael; Brummer, Janine; Gullick, Darren; Mckeever, Tricia M.; Whittaker, Mark A.; Nitch-Smith, Harriet; Brown, James F.; Shute, Janis K.; Mills, Graham; Calder, Philip C.; Trebble, Timothy M.

In: Journal of Crohn's and Colitis, Vol. 8, No. 1, 01.01.2014, p. 70-79.

Research output: Contribution to journalArticlepeer-review

Harvard

Pearl, DS, Masoodi, M, Eiden, M, Brummer, J, Gullick, D, Mckeever, TM, Whittaker, MA, Nitch-Smith, H, Brown, JF, Shute, JK, Mills, G, Calder, PC & Trebble, TM 2014, 'Altered colonic mucosal availability of n-3 and n-6 polyunsaturated fatty acids in ulcerative colitis and the relationship to disease activity', Journal of Crohn's and Colitis, vol. 8, no. 1, pp. 70-79. https://doi.org/10.1016/j.crohns.2013.03.013

APA

Pearl, D. S., Masoodi, M., Eiden, M., Brummer, J., Gullick, D., Mckeever, T. M., Whittaker, M. A., Nitch-Smith, H., Brown, J. F., Shute, J. K., Mills, G., Calder, P. C., & Trebble, T. M. (2014). Altered colonic mucosal availability of n-3 and n-6 polyunsaturated fatty acids in ulcerative colitis and the relationship to disease activity. Journal of Crohn's and Colitis, 8(1), 70-79. https://doi.org/10.1016/j.crohns.2013.03.013

Vancouver

Pearl DS, Masoodi M, Eiden M, Brummer J, Gullick D, Mckeever TM et al. Altered colonic mucosal availability of n-3 and n-6 polyunsaturated fatty acids in ulcerative colitis and the relationship to disease activity. Journal of Crohn's and Colitis. 2014 Jan 1;8(1):70-79. https://doi.org/10.1016/j.crohns.2013.03.013

Author

Pearl, Daniel S. ; Masoodi, Mojgan ; Eiden, Michael ; Brummer, Janine ; Gullick, Darren ; Mckeever, Tricia M. ; Whittaker, Mark A. ; Nitch-Smith, Harriet ; Brown, James F. ; Shute, Janis K. ; Mills, Graham ; Calder, Philip C. ; Trebble, Timothy M. / Altered colonic mucosal availability of n-3 and n-6 polyunsaturated fatty acids in ulcerative colitis and the relationship to disease activity. In: Journal of Crohn's and Colitis. 2014 ; Vol. 8, No. 1. pp. 70-79.

Bibtex

@article{13d33e3a77d64124b818fb7445e26fa5,
title = "Altered colonic mucosal availability of n-3 and n-6 polyunsaturated fatty acids in ulcerative colitis and the relationship to disease activity",
abstract = "Background and Aims The polyunsaturated fatty acids (PUFA) arachidonic acid (AA, n-6) and eicosapentaenoic acid (EPA, n-3) are precursors of eicosanoids and other lipid mediators which have critical roles in inflammation. The mediators formed from the different PUFA have different potencies. We hypothesised that metabolic changes associated with colonic mucosal inflammation would modify the bioavailability of the eicosanoid precursors AA and EPA. Methods Colonic mucosa biopsies were obtained from patients with ulcerative colitis and from matched controls. Inflammation was graded endoscopically and histologically. Esterified and non-esterified fatty acids were determined within the biopsies using gas chromatography–mass spectrometry and liquid chromatography–mass spectrometry, respectively. Results Biopsy samples were collected from 69 UC patients (54 providing both inflamed and non-inflamed mucosa) and 69 controls. Inflamed mucosa had higher AA (p < 0.001) and lower EPA (p < 0.010) contents and a higher AA:EPA ratio (p < 0.001). Inflamed mucosa also had higher docosapentaenoic acid (DPA) and docosahexaenoic acid (DHA) and lower linoleic acid (LA) and α-linolenic acid (α-LNA) contents (all p < 0.001), compared to non-inflamed and controls. There were significant correlations between severity of inflammation and contents of AA, DPA and DHA (positive correlations) and of LA, α-LNA and EPA (negative correlations). Conclusions Higher AA, AA:EPA ratio, DPA and DHA and lower LA, α-LNA and EPA are seen in inflamed mucosa in UC and correlate with severity of inflammation. This suggests an alteration in fatty acid metabolism in the inflamed gut mucosa, which may offer novel targets for intervention and should be considered if nutritional strategies are used.",
author = "Pearl, {Daniel S.} and Mojgan Masoodi and Michael Eiden and Janine Brummer and Darren Gullick and Mckeever, {Tricia M.} and Whittaker, {Mark A.} and Harriet Nitch-Smith and Brown, {James F.} and Shute, {Janis K.} and Graham Mills and Calder, {Philip C.} and Trebble, {Timothy M.}",
year = "2014",
month = jan,
day = "1",
doi = "10.1016/j.crohns.2013.03.013",
language = "English",
volume = "8",
pages = "70--79",
journal = "Journal of Crohn's and Colitis",
issn = "1873-9946",
publisher = "Elsevier",
number = "1",

}

RIS

TY - JOUR

T1 - Altered colonic mucosal availability of n-3 and n-6 polyunsaturated fatty acids in ulcerative colitis and the relationship to disease activity

AU - Pearl, Daniel S.

AU - Masoodi, Mojgan

AU - Eiden, Michael

AU - Brummer, Janine

AU - Gullick, Darren

AU - Mckeever, Tricia M.

AU - Whittaker, Mark A.

AU - Nitch-Smith, Harriet

AU - Brown, James F.

AU - Shute, Janis K.

AU - Mills, Graham

AU - Calder, Philip C.

AU - Trebble, Timothy M.

PY - 2014/1/1

Y1 - 2014/1/1

N2 - Background and Aims The polyunsaturated fatty acids (PUFA) arachidonic acid (AA, n-6) and eicosapentaenoic acid (EPA, n-3) are precursors of eicosanoids and other lipid mediators which have critical roles in inflammation. The mediators formed from the different PUFA have different potencies. We hypothesised that metabolic changes associated with colonic mucosal inflammation would modify the bioavailability of the eicosanoid precursors AA and EPA. Methods Colonic mucosa biopsies were obtained from patients with ulcerative colitis and from matched controls. Inflammation was graded endoscopically and histologically. Esterified and non-esterified fatty acids were determined within the biopsies using gas chromatography–mass spectrometry and liquid chromatography–mass spectrometry, respectively. Results Biopsy samples were collected from 69 UC patients (54 providing both inflamed and non-inflamed mucosa) and 69 controls. Inflamed mucosa had higher AA (p < 0.001) and lower EPA (p < 0.010) contents and a higher AA:EPA ratio (p < 0.001). Inflamed mucosa also had higher docosapentaenoic acid (DPA) and docosahexaenoic acid (DHA) and lower linoleic acid (LA) and α-linolenic acid (α-LNA) contents (all p < 0.001), compared to non-inflamed and controls. There were significant correlations between severity of inflammation and contents of AA, DPA and DHA (positive correlations) and of LA, α-LNA and EPA (negative correlations). Conclusions Higher AA, AA:EPA ratio, DPA and DHA and lower LA, α-LNA and EPA are seen in inflamed mucosa in UC and correlate with severity of inflammation. This suggests an alteration in fatty acid metabolism in the inflamed gut mucosa, which may offer novel targets for intervention and should be considered if nutritional strategies are used.

AB - Background and Aims The polyunsaturated fatty acids (PUFA) arachidonic acid (AA, n-6) and eicosapentaenoic acid (EPA, n-3) are precursors of eicosanoids and other lipid mediators which have critical roles in inflammation. The mediators formed from the different PUFA have different potencies. We hypothesised that metabolic changes associated with colonic mucosal inflammation would modify the bioavailability of the eicosanoid precursors AA and EPA. Methods Colonic mucosa biopsies were obtained from patients with ulcerative colitis and from matched controls. Inflammation was graded endoscopically and histologically. Esterified and non-esterified fatty acids were determined within the biopsies using gas chromatography–mass spectrometry and liquid chromatography–mass spectrometry, respectively. Results Biopsy samples were collected from 69 UC patients (54 providing both inflamed and non-inflamed mucosa) and 69 controls. Inflamed mucosa had higher AA (p < 0.001) and lower EPA (p < 0.010) contents and a higher AA:EPA ratio (p < 0.001). Inflamed mucosa also had higher docosapentaenoic acid (DPA) and docosahexaenoic acid (DHA) and lower linoleic acid (LA) and α-linolenic acid (α-LNA) contents (all p < 0.001), compared to non-inflamed and controls. There were significant correlations between severity of inflammation and contents of AA, DPA and DHA (positive correlations) and of LA, α-LNA and EPA (negative correlations). Conclusions Higher AA, AA:EPA ratio, DPA and DHA and lower LA, α-LNA and EPA are seen in inflamed mucosa in UC and correlate with severity of inflammation. This suggests an alteration in fatty acid metabolism in the inflamed gut mucosa, which may offer novel targets for intervention and should be considered if nutritional strategies are used.

U2 - 10.1016/j.crohns.2013.03.013

DO - 10.1016/j.crohns.2013.03.013

M3 - Article

VL - 8

SP - 70

EP - 79

JO - Journal of Crohn's and Colitis

JF - Journal of Crohn's and Colitis

SN - 1873-9946

IS - 1

ER -

ID: 217719