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Confirming antibody specificity in Xenopus

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Confirming antibody specificity in Xenopus. / Martin, Sian A.; Page, Suzannah J.; Piccinni, Maya; Guille, Matt.

8 p. 12 ed. Cold Spring Harbor Laboratory Press. 2020, Protocol. (Cold Spring Harbor Protocols).

Research output: Other contribution

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Martin, Sian A. ; Page, Suzannah J. ; Piccinni, Maya ; Guille, Matt. / Confirming antibody specificity in Xenopus. 2020. Cold Spring Harbor Laboratory Press. 8 p. (Cold Spring Harbor Protocols).

Bibtex

@misc{80838c9fd3ec429ba288e2d09825fc0e,
title = "Confirming antibody specificity in Xenopus",
abstract = "Verifying that a new antibody recognizes its target can be difficult. In this protocol, expression of a target protein in Xenopus embryos is either knocked down using CRISPR–Cas9 technology (for zygotic proteins) or enhanced by microinjection of a synthetic mRNA (for maternal proteins). Western blotting analysis is then performed. If the antibody recognizes the target protein, the western blot will show a relatively weak band for CRISPR-injected embryos and a relatively strong band for RNA-injected embryos. This represents a straightforward, powerful strategy for confirming antibody specificity in Xenopus.",
author = "Martin, {Sian A.} and Page, {Suzannah J.} and Maya Piccinni and Matt Guille",
year = "2020",
month = dec,
day = "1",
doi = "10.1101/pdb.prot105601",
language = "English",
volume = "2020",
series = "Cold Spring Harbor Protocols",
publisher = "Cold Spring Harbor Laboratory Press",
address = "United States",
edition = "12",
type = "Other",

}

RIS

TY - GEN

T1 - Confirming antibody specificity in Xenopus

AU - Martin, Sian A.

AU - Page, Suzannah J.

AU - Piccinni, Maya

AU - Guille, Matt

PY - 2020/12/1

Y1 - 2020/12/1

N2 - Verifying that a new antibody recognizes its target can be difficult. In this protocol, expression of a target protein in Xenopus embryos is either knocked down using CRISPR–Cas9 technology (for zygotic proteins) or enhanced by microinjection of a synthetic mRNA (for maternal proteins). Western blotting analysis is then performed. If the antibody recognizes the target protein, the western blot will show a relatively weak band for CRISPR-injected embryos and a relatively strong band for RNA-injected embryos. This represents a straightforward, powerful strategy for confirming antibody specificity in Xenopus.

AB - Verifying that a new antibody recognizes its target can be difficult. In this protocol, expression of a target protein in Xenopus embryos is either knocked down using CRISPR–Cas9 technology (for zygotic proteins) or enhanced by microinjection of a synthetic mRNA (for maternal proteins). Western blotting analysis is then performed. If the antibody recognizes the target protein, the western blot will show a relatively weak band for CRISPR-injected embryos and a relatively strong band for RNA-injected embryos. This represents a straightforward, powerful strategy for confirming antibody specificity in Xenopus.

U2 - 10.1101/pdb.prot105601

DO - 10.1101/pdb.prot105601

M3 - Other contribution

VL - 2020

T3 - Cold Spring Harbor Protocols

PB - Cold Spring Harbor Laboratory Press

ER -

ID: 25019862